We aimed to determine whether shotgun proteomic approaches could be used to identify tuberculosis (TB)-specific biomarkers in the urine of well-characterised patients with active TB versus no TB. Patients with suspected TB (n=63) were classified as: definite TB (Mycobacterium tuberculosis positive culture, n=21); presumed latent-TB infection (LTBI) (M. tuberculosis negative culture, no radiolog...

CONTEXT Although tuberculosis (TB) is the leading worldwide cause of death due to an infectious disease, the extent to which progressive clinical disease is associated with genetic host factors remains undefined. OBJECTIVE To determine the distribution of HLA antigens and the frequency of 2 alleles of the tumor necrosis factor alpha (TNF-alpha) gene in unrelated individuals with clinical TB (...

Biomarker host genetic signatures are considered key tools for improved early diagnosis of tuberculosis (TB) disease (development). The analysis of gene expression changes based on a limited number of genes or single study designs, however, may not be sufficient for the identification of universal diagnostic biomarker profiles. Here we propose that biological pathway and process based analyses ...

background and objective: molecular epidemiological studies have shown that certain genotypes of mycobacterium tuberculosis (mtb) are over-represented in limited geographical regions, suggesting of evolution of certain genotypes with increasing virulence and pathogenicity. beijing strain of mtb was initially described by its potential to cause outbreaks worldwide and its association with drug r...

BACKGROUND Chemokine (C-C motif) ligand 2 CCL2/MCP-1 is among the key signaling molecules of innate immunity; in particular, it is involved in recruitment of mononuclear and other cells in response to infection, including tuberculosis (TB) and is essential for granuloma formation. METHODOLOGY/PRINCIPAL FINDINGS We identified a tag SNP for the CCL2/MCP-1 gene (rs4586 C/T). In order to understa...

There are differences in probe pattern missing rifampicin resistance determining region of rpoB gene among primary and acquired resistant TB cases. This study aimed to observe the Xpert® MTB RIF Assay presenting Hotspot RRDR Gene drug-resistant cases pulmonary tuberculosis a tertiary care setting Lahore. comparative cross-sectional work was undertaken at The University Lahore cooperation with D...

Tuberculosis (TB), an infectious disease caused by infection of Mycobacterium tuberculosis, is a major public health challenge globally. Genetic epidemiological evidence suggests a genetic basis for TB, but the molecular mechanism for a genetic predisposition to TB remains largely unknown. Thirty-five tag single-nucleotide polymorphisms (SNPs) across 11 candidate cytokines and related genes, in...

BACKGROUND & OBJECTIVES Variations in the N-acetyltransferase (NAT2) gene among different populations could affect the metabolism and disposition of isoniazid (INH). This study was performed to genotype NAT2 gene polymorphisms in tuberculosis (TB) patients from Chennai, India, and compare plasma INH concentrations among the different genotypes. METHODS Adult patients with TB treated in the Re...

Subacute invasive pulmonary aspergillosis is a form of chronic (CPA) with rapid progression. The clinical features CPA mimic tuberculosis (TB) and may lead to delayed and/or misdiagnosis. We report 39-year-old Nigerian previously managed in peripheral hospital as case TB despite negative Gene-X pert results no resolution symptoms. Chest X-ray computer tomography findings were suggestive galacto...