BACKGROUND We assessed the maturational competence and the chromosomal pattern of mouse oocytes reconstructed by germinal vesicle (GV) transfer technique using nuclear and/or cytoplasmic components from cryopreserved GV stage oocytes. METHODS From 657 GV oocytes (326 fresh and 331 frozen/thawed), four groups of reconstructed oocytes were obtained by micromanipulation and electrofusion: fresh ...

The regulation of the cell cycle during the transition from prophase to metaphase I was studied by analysing protein changes and introducing protein blocks during the transition phase. The results show that the progression to metaphase in ovine oocytes is totally dependent on new protein synthesis. By delaying the addition of the inhibitor, cycloheximide, for progressively longer periods after ...

The nucleus of eukaryotic cells is organized into functionally specialized compartments that are essential for the control of gene expression, chromosome architecture and cellular differentiation. The mouse oocyte nucleus or germinal vesicle (GV) exhibits a unique chromatin configuration that is subject to dynamic modifications during oogenesis. This process of 'epigenetic maturation' is critic...

The Rockefeller University Press $30.00 J. Cell Biol. Vol. 208 No. 1 53–69 www.jcb.org/cgi/doi/10.1083/jcb.201404109 JCB 53 Correspondence to Rabindranath De La Fuente: [email protected]; or John C. Schimenti: [email protected] Abbreviations used in this paper: ACT, activator of CREM in testis; CREM, cAMP response element modulator; GV, germinal vesicle; IAP, intracisternal A particle; MGI, Mouse...

Background: in vitro maturation of human germinal oocytes is a promising technique assisted reproductive technologies, different medium has been introducing last two decays for this purpose but they are very expensive and gained by order with qualification Objective: the aim study to evaluate efficacy fertilization used vesicles during ICSI cycles. Patients methods: 42 vesicle stage involved (I...

We report on a binuclear human oocyte that underwent maturation in vitro from germinal vesicle stage to metaphase II. Following extrusion of two polar bodies, the oocyte was processed for cytogenetic analysis which revealed two separate haploid chromosome sets accompanied by the corresponding polar body chromatin. Tentatively established karyotypes were 23,X and 23,X,ace, respectively. This con...

We prepared hybridoma cell lines from mice injected with newt germinal vesicle proteins. We tested culture supernates from these cell lines for antibodies that bound to specific morphological structures in lampbrush chromosome preparations (nucleoli, loops, chromomeres, etc.). Four mAbs that recognize antigens on the lateral transcription loops are described here. We suggest that these antigens...