Abbreviations: Ab, amyloid-b; AD, Alzheimer disease; APLP, amyloid precursor–like protein; APP, amyloid precursor protein; CaMKIIa, calciumcalmodulin kinase IIa; dox, doxycycline; FA, formic acid; GFAP, glial fibrillary acidic protein; mo/ huAPP695, mouse APP with a humanized Ab domain; PBS, phosphate-buffered saline; SDS, sodium dodecyl sulfate; swe/ind, Swedish/Indiana; tTA, tetracycline tran...

Neuronal gene expression is known to be modulated by functional activity. This modulation is thought to play a key role in determining the differentiation of developing neurons and regulating the operation of mature neurons. Here we describe a regulation of astroglial gene expression by neuronal activity. We report that intense neuronal activity (electrically induced seizures) in rat hippocampu...

The immunohistochemical expression of glial fibrillary acidic protein (GFAP) and vimentin (VIM) was studied in reactive astrocytes of the rat cerebral cortex 5 days after a brain injury. Seriated Epon semithin sections were immunostained alternatively for GFAP or VIM. Thereafter, both antigens were detected in consecutive sections of the same cell. Bordering the wound, an inner reactive glial l...

Glial cells play a critical role in morphine tolerance, resulting from repeated administration of morphine. Both the development and the expression of tolerance are suppressed by the analgesic lamotrigine. This study investigated the relationship between the ability of lamotrigine to maintain the antinociceptive effect of morphine during tolerance development and glial cell activation in the sp...

GFAP-TK mice are widely used in studies on neurogenesis and reactive astrocytes. Previous studies reported that GCV treatment in GFAP-TK mice resulted in reduced neurogenesis and deletion of proliferating GFAP-expressing astrocytes without affecting mature GFAP-expressing astrocytes. In the present study, we found that GFAP- and vimentin-expressing astrocytes were dramatically increased in the ...

The presence and distribution of glial fibrillary acidic protein, vimentin, neurofilament protein, cytokeratins No. 8 (52 Kd), No. 18 (45 Kd) and No. 19 (40 Kd) and S-100 protein in pituicytes, folliculo-stellate cells, the epithelium of the Rathke's cysts and squamous cell nests of the pars tuberalis were investigated immunohistochemically by the peroxidase-antiperoxidase (PAP) method in eleve...

A case of cerebellar medulloblastoma with transitional features towards malignant fibrillary astrocytoma is described. In the cerebellum the tumour is characterised by extensive subpial infiltration with cells of undifferentiated type, and the astrocytic component could only be identified by a positive glial fibrillary acidic protein reaction. In the brainstem the character of the growth transf...