Dardas, T. J. (Michigan State University, East Lansing), and V. H. Mallmann. Electrophoretic and immunoelectrophoretic studies of sera from normal, tuberculous, and noninfected tuberculin-sensitive guinea pigs. J. Bacteriol. 92:76-81. 1966.-Normal guinea pig serum was separated into seven fractions by electrophoresis on cellulose acetate membranes. Thirty antigens were found by immunoelectropho...

The progress of an infection is usually associated with marked changes in the serum proteins. There may be an increase in the percentage of the total protein during some stage of the infection, and there is usually a change in the albumin-globulin ratio with an increase in the total globulins. This rise may antedate the development of any resistance by a considerable period of time. The non-pro...

THE RESULTS OF THE FOREGOING EXPERIMENTS MAY BE BRIEFLY SUMMARIZED AS FOLLOWS: The amount of antitoxic substance obtained by precipitation with magnesium sulphate from the blood-serum of the horse corresponds, as nearly as can be determined by the use of test guinea-pigs, in full to the protective power of the serum from which it is obtained, i. e. the precipitate from 1 cc. of serum will prote...

A decreased A/G ratio may indicate multiple myeloma, autoimmune disease, cirrhosis, or kidney disease. The patient’s electrophoretogram was normal, and subsequent immunofixation was negative, which ruled out monoclonal gammopathy. The SPE (Fig. 1) showed an albumin of 64.4% and normal A/G ratio, which suggests spurious hyperproteinemia. The patient was being treated with dextran (1 ). In the as...

The use of intravenous immunoglobulin (IVIg), which is immunoglobulin G pooled from thousands of healthy donors, in the treatment of immunodeficient and autoimmune diseases has grown during the past 2 decades. Although its initial application was largely limited to replacement therapy in hypogammaglobulinemia, IVIg is gaining acceptance as therapy for autoimmune thrombocytopenia purpura, and a ...

Antinuclear antibodies were found in 64% of serum samples obtained from male NZB/NZW mice after 6 weeks of treatment with mestranol. Both female and male mice responded to this synthetic female hormone with marked elevations of serum “2’ and p-globulins; 7-globulins were not increased. There was no evidence that mestranol accelerated the develop ment of autoimmune renal disease in these animals...

A sensitive and rapid ELISA for quantitation of seed globulins is described. This method employs conjugation of pigeon pea (Cajanus cajan) globulin antibodies and the enzyme peroxidase together with dextran. Using this conjugate, proteins as low as 0.1 ng were detected. Dextran conjugate has a ten-fold greater efficiency of quantitating pigeon pea globulins than the commercial goat anti-rabbit ...