In the framework of artificial or synthetic cell development, giant liposomes are common basic structures. Their enclosed membrane allows encapsulating proteins, DNA, reactants, etc., while its phospholipid nature some exchanges with surrounding medium. Biochemical reactions induced inside vesicles often monitored imaged by fluorescence microscopy techniques. Here, we show that electrochemistry...

A flow cell has been designed for use with an electrochemical enzyme biosensor, based on low-cost carbon-film electrodes. Three types of mediators were used: cobalt and copper hexacyanoferrates and poly(neutral red) (PNR), covered with glucose oxidase (GOx) immobilised by cross-linking with glutaraldehyde in the presence of bovine serum albumin or inside a oxysilane sol-gel network. Mixtures of...

A glucose biosensor based on a nanocomposite made by layer-by-layer electrodeposition of the redox polymer into a multilayer containing glucose oxidase (GOx) and single-walled carbon nanotubes (SWCNT) on a screen-printed carbon electrode (SPCE) surface was developed. The objectives of the electrodeposition of redox polymer are to stabilize further the multilayer using a coordinative cross-linke...

The Glucose Oxidase (Gox) electrochemical sensors were used to detect a little amount of glucose. In this paper, the pure platinum plate (length of 5 cm and a width of 3 mm), the Ag/AgCl electrode and platinum electrode (diameter of 1 mm) were applied as a working electrode (W.E), reference electrode and auxiliary electrode sequential. Factors measured, glucose concentration and pH is Phosphate...

The Glucose Oxidase (Gox) electrochemical sensors were used to detect a little amount of glucose. In this paper, the pure platinum plate (length of 5 cm and a width of 3 mm), the Ag/AgCl electrode and platinum electrode (diameter of 1 mm) were applied as a working electrode (W.E), reference electrode and auxiliary electrode sequential. Factors measured, glucose concentration and pH is Phosphate...

The increasing demand in studying cellular functions in cultured cells under various levels of oxygen and hydrogen peroxide (H2O2) is only partly fulfilled by conventional approaches such as hypoxia chambers, bolus additions of H2O2 or redox-cycling drugs. This article describes the recently developed enzymatic GOX/CAT system consisting of glucose oxidase (GOX) and catalase (CAT) that allows th...

: A simple procedure was developed for the fabrication of electrochemical glucose biosensors using glucose oxidase (GOx), with graphene or multi-walled carbon nanotubes (MWCNTs). Graphene and MWCNTs were dispersed in 0.25% 3-aminopropyltriethoxysilane (APTES) and drop cast on 1% KOH-pre-treated glassy carbon electrodes (GCEs). The EDC (1-ethyl-(3-dimethylaminopropyl) carbodiimide)-activated GOx...

Amperometric biosensors based on the use of glucose oxidase (GOx) are able to combine the robustness of electrochemical techniques with the specificity of biological recognition processes. However, very little information can be found in literature about the fundamental analytical parameters of these sensors. In this work, the analytical behavior of an amperometric biosensor based on the immobi...

In an ongoing programme to develop characterization strategies relevant to biosensors for in-vivo monitoring, glucose biosensors were fabricated by immobilizing the enzyme glucose oxidase (GOx) on 125 μm diameter Pt cylinder wire electrodes (Pt(C)), using three different methods: before, after or during the amperometric electrosynthesis of poly(ortho-phenylenediamine), PoPD, which also served a...

Many researchers investigating plant-insect interactions maintain their insect colonies on artificial diet and assume that salivary enzymes and elicitors remain representative of natural situations. These salivary elicitors, such as the enzyme glucose oxidase (GOX), play important roles in influencing plant defense responses. In fact, GOX has been implicated in suppressing induced nicotine-prod...