Interactions across biological networks are often quantified under a single set of conditions; however, cellular behaviors are dynamic and interactions can be expected to change in response to molecular context and environment. To determine the consistency of network interactions, we examined the enzyme network responsible for the reduction of nicotinamide adenine dinucleotide phosphate (NADP) ...

One hundred and sixty-nine Javanese males were screened for the presence of red cell glucose-6-phosphate dehydrogenase (G6PD) variants by a dye decoloration screening test and starch gel electrophoresis. The frequency of G6PD deficiency was 14%. Three non-deficient electrophoretic variants with mobilities of 95, 105 and 107% of GdB+ were encountered. Sixteen G6PD-deficient subjects were further...

The human X chromosome-linked gene encoding glucose-6-phosphate dehydrogenase (G6PD; EC 1.1.1.49) is known to be highly polymorphic from the biochemical characterization of enzyme variants. The variant A (with enzyme activity in the normal range) and the variant A- (associated with enzyme deficiency) each have a frequency of about 0.2 in several African populations. Two restriction fragment len...

I NVESTJGATIONS OF RED CELL GLUCOSE-6-PHOSPHATE DEHYDROGENASE ( D-glucose-6-phosphate: NADP oxidoreductase, EC 1.1.1.49; G6PD ) in different human populations have shown that more than 50 variants exist at this time.1’2 Genetic studies are compatible with X-linkage of all G6PD variants. Some variants are associated with normal enzyme activity and therefore are not accompanied by any clinical ma...

The prevalence of glucose-6-phosphate dehydrogenase (G6PD) deficiency is high in Asia. An ex vivo study was conducted to elucidate the association of G6PD deficiency and dengue virus (DENV) infection when many Asian countries are hyper-endemic. Human monocytes from peripheral mononuclear cells collected from 12 G6PD-deficient patients and 24 age-matched controls were infected with one of two DE...

Glucose-6-phosphate dehydrogenase deficiency is a common hemolytic genetic disorder, particularly in the areas endemic to malaria. The risk of drug induced Glucose-6-phosphate dehydrogenase deficiency related hemolysis depends on a number of factors including the Glucose-6-phosphate dehydrogenase variant. Aims: To know the G6PD deficiency and identify its variants in drug induced hemolysis in m...

An automated fluorometric microtechnic, suitable for screening purposes, has been developed for the quantitative determination of glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) levels in red blood cells. The method is rapid, simple, and precise. It uses 50 p.I whole-blood (cpillary) samples and is based on the measurement of natural fluorescence emitted by ...

Insulin-like material can be extracted from insulin-Sepharose. This soluble material elicits super-insulin responses from mammary epithelial cells. It stimulates the accumulation of alpha-aminoisobutyric acid by virgin mouse mammary cells, which are unresponsive to insulin itself. It exerts a greater stimulatory effect on mammary cells from pregnant mice than insulin. It also is more efficaciou...

Seasonal patterns of allozyme variation are examined for 12 polymorphic enzyme loci in Drosophila melanogaster. The data derive from a total of 56 samples taken from a natural population in the Summer and Fall of 1978 and 1979. Samples were obtained at approximately five-day intervals and assayed for 6-phosphogluconate dehydrogenase (6Pgd), phosphoglucomutase (Pgm) and glucose-6-phosphate dehyd...

It is now well established that hyperbilirubinaemia and even kernikterus may occur in the newborn in association with glucose 6 phosphate dehydrogenase (G6PD) deficiency, in the absence of any of the other causes of neonatal jaundice (Panizon, 1960a, b; Doxiadis, Fessas, and Valaes, 1960; Doxiadis, Fessas, Valaes, and Mastrokalos, 1961; Fessas, Doxiadis, and Valaes, 1962; Smith and Vella, 1960;...