Methods using dialysis or ultrafiltration are described for the collection of extracellular fluid in rumen contents for analysis of amino acids. Marked differences in the concentration of aspartic acid, glutamic acid, and alanine were found in samples of either diffusate or ultrafiltrate and in clarified acidified rumen liquor. Concentrations are given for aspartic acid, glutamic acid, alanine,...

A fragmentation mechanism for the neutral loss of 73 Da from dimethylformamidine glutamine isobutyl ester is investigated. Understanding this mechanism will allow to improve the identification and quantification of 15N-labeled and unlabeled glutamine and the distinguishing of glutamine and glutamic acid by electrospray ionization (ESI)-tandem mass spectrometry. Before mass spectrometry analysis...

To clarify the relationship between intracellular concentrations of methylmalonic acid and metabolic and growth inhibition in vitamin B12-deficient rats, hepatic methylmalonic acid levels were assayed and inhibition of glucose and glutamic acid metabolism by methylmalonic acid was studied in isolated hepatocytes. Vitamin B12-deficient rats (14 weeks old) excreted more urinary methylmalonic acid...

In preceding reports from this laboratory, kyneurenic acid was shown to be degraded by cell-free extracts of Pseudomonas Jluorescens (ATCC 11299B) with the formation of L-glutamic acid, Dand L-alanine, and acetic acid as the major end products (2, 3). Subsequently, tracer experiments with kynurenic acid labeled with Cl4 at various positions demonstrated that the carbon skeleton of glutamic acid...

In 1932, Quastel and Wheatley (1) measured oxygen consumption in chopped brain tissue to which various metabolites had been added. Glutamic acid was oxidized at a relatively slow rate. According to Krebs (2), who repeated and extended these studies, L-glutamic acid was the only amino acid which was capable of maintaining the respiration of slices of brain or retina. This observation was confirm...

Hayashi1) reported that a convulsive seizure can be induced on dogs, by injecting a very minute amount of sodium salt of glutamic acid into the cortex of the brain. We also observed a similar phenomenon caused by injecting the drug into the brain or the spinal cord of a frog or a toad. The present experiment was undertaken in order to investigate the effect of glutamic acid upon the potentials ...

Methionine sulfoxide has been shown to be a specific antagonist of glutamic acid in the conversion of glutamic acid to glutamine by Waelsch, Owades, Miller, and Borek (J. Biol. Chem., 166, 273, 1946) in growth inhibition studies with Lactobacillus arabinosus. Elliot and Gale (Nature, 161, 129, 1948) using cell-free extracts of Staphylococcus aureus have demonstrated that methionine sulfoxide in...

The rates of incorporation of ^C-labeled glycine and glutamic acid into glutathione and ophthalmic acid have been determined in cultured rabbit lenses. The average turnover rate of glycine and glutamic acid in glutathione, and of glutamic acid in ophthalmic acid teas found to be 1.8 per cent per hour throughout the 24 hour period of culture; the incorporation of glycine in ophthalmic acid also ...