Addition of 2-deoxyglucose to a culture of the hybrid yeast Saccharomyces fragilis X Saccharomyces dobzhanskii causes 2to 200-fold increase in the differential rate of synthesis of all glycolytic enzymes except alcohol dehydrogenase (EC 1.1.1.1). For most enzymes, the stimulation in the rate of synthesis occurs with a delay. The greatest stimulation is observed in the case of glyceraldehyde39 d...

waterlogging is one of the problems of cotton-growing in rainy regions which affects adversely plant growth in the early stages. so that rainfall causes waterlogging in early to mid spring in the fields without proper drainage. also, rainfall declines soil and environment temperatures. to investigate the effects of waterlogging period duration and temperature on vegetative properties and glycol...

Resting rat thymocytes were stimulated to cell cycle entry and proliferation by the addition of concanavalin A and interleukin 2 to the culture medium. Maximal rats of DNA and RNA synthesis and of glycolysis with a 20-fold increase in glucose uptake were observed 48 h after stimulation. Glycolytic enzyme levels increased 3-8-fold, also reaching their maxima 48 h after mitogenic stimulation. Act...

The glycolytic pathway of the hyperthermophilic archaea that belong to the order Thermococcales (Pyrococcus, Thermococcus and Palaeococcus) differs significantly from the canonical Embden–Meyerhof pathway in bacteria and eukarya. This archaeal glycolysis variant consists of several novel enzymes, some of which catalyze unique conversions. Moreover, the enzymes appear not to be regulated alloste...

AIMS Cardiomyocytes (CMs) and endothelial cells (ECs) have an intimate anatomical relationship, which is essential for maintaining the metabolic requirements of the heart. Little is known about the mechanisms that regulate nutrient flow from ECs to associated CMs, especially in situations of acute stress when local active processes are required to regulate endothelial transport. We examined whe...

The understanding of the effective functionality that governs the enzymatic self-organized processes in cellular conditions is a crucial topic in the post-genomic era. In recent studies, Transfer Entropy has been proposed as a rigorous, robust and self-consistent method for the causal quantification of the functional information flow among nonlinear processes. Here, in order to quantify the fun...

Phosphoglycerate mutases catalyze the interconversion of 2- and 3-phosphoglycerate in the glycolytic and gluconeogenic pathways. They exist in two unrelated forms that are either cofactor (2,3-diphosphoglycerate)-dependent or cofactor-independent. The two enzymes have no similarity in amino acid sequence, tertiary structure, or catalytic mechanism. Certain organisms including vertebrates have o...

The course of bacterial invasioni of a particular tissuie varies according to a variety of factors suclh as synergic or antagonist actions between invaders, nonspecific and specific lhost responses, and alterations in the host environment. Anotlher dleterminant of the extent of a particular tissue involvement is the striking tendency of microorganisms to invade specific cells or to localize in ...

A summary of a survey of three genera of mycoplasmatales (Mycoplasma, Acholeplasma, and Ureaplasma) for isozyme expression is presented. Isozyme analysis of mycoplasmas has been employed in at least three distinct areas: (1) as genetic markers for identification, individualization, and taxonomic classification; (2) as markers for cell culture contamination; and (3) as a qualitative measure of t...