OBJECTIVE Lipid hydroperoxide, a marker of oxidative stress, is linked to the development of nephropathy and is reportedly higher in patients of African origin compared with Caucasians. This may be relevant to race-specific differences in susceptibility to nephropathy. We investigated whether alterations in antioxidant enzyme activity could account for this biochemical phenotype and examined th...

Multi-trial Lin-Kernighan-Helsgaun 2 (LKH-2) is widely considered to be the best Interated Local Search heuristic for the Traveling Salesman Problem (TSP) and has found the best-known solutions to a large number of benchmark problems. Although LKH-2 performs exceptionally well on most instances, it is known to have difficulty on clustered instances of the TSP. Generalized Partition Crossover (G...

Superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione S-transferase (GST) and glutathione reductase (GR) play crucial roles in balancing the production and decomposition of reactive oxygen species (ROS) in living organisms. These enzymes act cooperatively and synergistically to scavenge ROS, as not one of them can singlehandedly clear all forms of ROS. In order to imitate the sy...

Background Oxidant injury to the pulmonary endothelium contributes to acute lung injury. We have shown that activation of PKGI by cGMP increases protein levels of the antioxidant enzymes catalase and glutathione peroxidase-1 (GPx-1) and ameliorates oxidant injury in mouse lung endothelium [1]. Catalase and GPx-1 mRNA was not increased. The pathway downstream of PKGI that leads to increases in c...

AIM To study the secretory activity and androgen regulation of glutathione peroxidase (GPx) in epithelial cell cultures from human epididymis. METHODS Tissue was obtained from patients undergoing therapeutic orchidectomy for prostatic cancer. Epithelial cell cultures were obtained from the caput, corpus and cauda epididymides. Enzymatic activity was measured in conditioned media by colorimetr...

Down syndrome or trisomy 21 is the single most common genetic cause of mental retardation. It has been postulated that there is oxidative stress in Down syndrome due to over expression of superoxide dismutase 1 (SOD -1) , an antioxidant enzyme which is coded on chromosome 21(21q22.1). The present study included 31 clinically diagnosed children with Down syndrome. The diagnosis of trisomy 21 was...

Superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT) play crucial roles in balancing the production and decomposition of reactive oxygen species (ROS) in living organisms. These enzymes act cooperatively and synergistically to scavenge ROS. In order to imitate the synergism of these enzymes, we designed and synthesized a novel 32-mer peptide (32P) on the basis of the prev...

Glutathione peroxidase (GPx) is an antioxidant protein containing selenium. Owing to the limitations of native GPx, considerable efforts have been made to develop GPx mimics. Here, a short 5-mer peptides (5P) was synthesized and characterized using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Enzyme coupled assays were used to evaluate GPx activity. The cell via...