Germ granules (P granules) in C. elegans are required for fertility and function to maintain germ cell identity and pluripotency. Sterility in the absence of P granules is often accompanied by the misexpression of soma-specific proteins and the initiation of somatic differentiation in germ cells. To investigate whether this is caused by the accumulation of somatic transcripts, we performed mRNA...

Purpose: To characterize some preformulation properties of aqueous stem bark extract of Bridelia ferruginea Benth (Euphorbiaceae) (BF). Methods: The stem bark was extracted by maceration in hot distilled water. Two batches of granules containing the extract were prepared by wet granulation using maize starch, polyvinylpyrrolidone (PVP), Avicel PH-101, magnesium stearate, acacia and lactose as e...

The present study was aimed at the development of aerobic granules in a sequencing batch reactor for the biodegradation of m-cresol. The reactor was started with 100 mg L(-1)of m-cresol. Aerobic granules first appeared within 1 month of the start-up of the reactor. The granules were large and strong and had a compact structure. The diameter of stable granules on day 200 was in the range of 1.5 ...

Previous reports on the ultrastructure of cartilage matrix have described fibers, amorphous ground substance and, in some instances, dense matrix granules. The fibers are presumably collagen, but the nature of the granules is unknown. The primary purpose of this study has been to investigate the ultrastructure of cartilage matrix ih chick embryos with particular emphasis on the distribution and...

The folded cortex of the growing oocyte of the frog extends as microvilli into the substance of the developing vitelline membrane and, internal to the folds, possesses a layer of cortical granules. Free ribosomes, smooth-walled vesicles, coated vesicles, tubules, and electron-opaque granules are abundant in the peripheral zone of the cortex. Mitochondria, lipochondria, pigment granules, and ele...

Neuromelanin is a complex polymer pigment found primarily in the dopaminergic neurons of human substantia nigra. Neuromelanin pigment is stored in granules including a protein matrix and lipid droplets. Neuromelanin granules are yet only partially characterised regarding their structure and function. To clarify the exact function of neuromelanin granules in humans, their enrichment and in-depth...

Sorting of RNAs to specific subcellular loci occurs in diverse settings from fly oocytes to mammalian neurons. Using the membrane-permeable nucleic acid stain SYTO 14, we directly visualized the translocation of endogenous RNA in living cells. Labeled RNA was distributed nonrandomly as discrete granules in neuronal processes. The labeled granules colocalized with poly(A+) mRNA, with the 60S rib...

We have examined the role of the R-soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) synaptobrevin-2/vesicle-associated membrane protein (VAMP)-2 in neutrophil exocytosis. VAMP-2, localized in the membranes of specific and gelatinase-containing tertiary granules in resting human neutrophils, resulted translocated to the cell surface following neutrophil activation un...

By Doms Kuirni, A. DEIss AND C. E. C wrwmcrr T HE OCCURRENCE of siderocytes in the blood of splenectomized patients, in the blood of some patients with hemolytic anemia, and particularly in splenectomized patients with persistent hemolytic anemia is well known.’5 More recently, as a result of experimental studies in swine,6” it has been recognized that the physiologic significance and metabolic...