Treatment of polycytidylic acid with hydroxylamine produces an inactivation of its template properties as measured by incorporation of guanosine 5’-phosphate. Partial recovery of the guanosine 5’-phosphate incorporation can be obtained by the addition of adenosine 5’-triphosphate, but not @dine 5’4riphosphate or uridine 5’4riphosphate. Nearest neighbor studies showed that the adenosine 5’-phosp...

An enzyme system from fruits of the flax plant is described that catalyzes the synthesis of the sugar nucleotide guanosine 5'-diphosphate l-fucose from guanosine 5'-diphosphate d-mannose with the intermediate formation of guanosine 5'-diphosphate 4-keto-6-deoxy-d-mannose. Tritium from-[(3)H]H(2)O was incorporated into the l-fucose portion of the sugar nucleotide in the course of the reaction, a...

phosphate intermediates, judging from lack of contribution to adenine, uracil, and cytosine nucleosides in RNA and DNA. The group of enzymatic activities that catalyze the conversion of guanine and guanosine to guanosine triphosphate (namely, guanosine kinase, purine nucleoside phosphorylase, purine nucleoside monophosphate kinase, nu cleoside diphosphate kinase, and purine nucleoside triphosph...

Guanosine-specific cyclic nucleotide signaling is suggested to serve protective actions in the vasculature; however, the influence of selective pharmacologic modulation of cyclic guanosine monophosphate- synthesizing soluble guanylate cyclase or cyclic guanosine monophosphate-degrading phosphodiesterase on vessel remodeling has not been thoroughly examined. In this study, rat carotid artery bal...

The existence of RNA enzymes that catalyze phosphodiester transfer reactions suggests that RNA-catalyzed RNA replication might be possible. Indeed, it has been shown that the Tetrahymena and sunY self-splicing introns will catalyze the template-directed ligation of RNA oligonucleotides (Doudna et al., 1989, 1991). We have sought to develop a more general RNA replication system in which arbitrar...

Silicate glasses are ubiquitous on terrestrial planets wherever molten rock – generated by volcanism or impacts is quenched air water. Hence, they provided ready and abundant reactive solid surfaces for prebiotic chemistry the early Earth. Here, we show that glass composition determines basicity, which in turn modulates outcome of synthetic processes such as polymerization 3’,5’ cyclic guanosin...

Biomolecules assemble to conduct: An ion conducting transparent hydrogel with thermo-reversibility, self-healing and injectable properties can be developed spontaneously through a supramolecular self-assembly of phytic acid guanosine monophosphate at room temperature. The efficient ionic mobility in the self-assembled crosslinked G-quadruplex nanofibres resulted its applicability as gel electro...