The ectodomain of the Ebola virus Gp2 glycoprotein was solubilized with a trimeric, isoleucine zipper derived from GCN4 (pIIGCN4) in place of the hydrophobic fusion peptide at the N terminus. This chimeric molecule forms a trimeric, highly alpha-helical, and very thermostable molecule, as determined by chemical crosslinking and circular dichroism. Electron microscopy indicates that Gp2 folds in...

A snake (of length L) is a (continuous) piecewise C1-curve S : [0, L] → R, parameterized by arc-length and whose “tail” is at the origin (S(0) = 0). Charming a snake consists in having it move in such a way that its “snout” S(L) follows a chosen C1-curve γ(t). The snake charmer algorithm, initiated in [Ha2] for polygonal snakes and developed in [Ro] in the general case, works as follows. The in...

Bessel beams are widely used in optical metrology mainly because of their large Rayleigh range (focal length). Radial/azimuthal polarization of such beams is of interest in the fields of material processing, plasma absorption or communication. In this paper an experimental set-up is presented, which generates a Bessel-type vector beam with a spatial polarization, oscillating along the optical a...

An H4N6 subtype avian influenza virus was isolated from a pockmark duck in southern China in November 2013 and named A/duck/Guangxi/149D24/2013 (H4N6). All eight gene segments of the strain were sequenced. Sequence analysis indicated that this H4N6 virus was a natural reassortant virus. This H4N6 virus has two basic amino acids in the cleavage site of hemagglutinin 1 (HA1) and HA2, and the amin...

This paper describes methods to produce an isotopically labeled 23 kDa viral membrane protein with purified yield of 20 mg/L of Esch­e­rich­ia coli shake flask culture. This yield is suf­fi­cient for NMR structural studies and the protein production methods are simple, straightforward, and rapid and likely applicable to other recombinant membrane proteins expressed in E. coli. The target FHA2 p...

A snake (of length L) is a (continuous) piecewise C1-curve S : [0, L] → R, parameterized by arc-length and whose “tail” is at the origin (S(0) = 0). Charming a snake consists in having it move in such a way that its “snout” S(L) follows a chosen C1-curve γ(t). The snake charmer algorithm, initiated in [Ha2] for polygonal snakes and developed in [Ro] in the general case, works as follows. The in...

Job satisfaction as a favorable or unfavorable situational condition and how employees view their work. The purpose of this study was to determine the effect job workload on employee performance at PT Inkabiz Indonesia partially, simultaneously. method used in conducting research is quantitative. results are has positive significant performance. Hypothesis test obtained value t count > table...