Skeletal development and invasion by tumor cells depends on proteolysis of collagen by the pericellular metalloproteinase MT1-MMP. Its hemopexin-like (HPX) domain binds to collagen substrates to facilitate their digestion. Spin labeling and paramagnetic nuclear magnetic resonance (NMR) detection have revealed how the HPX domain docks to collagen I-derived triple helix. Mutations impairing tripl...

In membrane proteins, proline-mediated helix kinks are indispensable for the tight packing of transmembrane (TM) helices. However, kinks invariably affect numerous interhelical interactions, questioning the acceptance of proline substitutions and evolutionary origin of kinks. Here, we present the structural and thermodynamic basis of proline-induced integrin αIIbβ3 TM complex stabilization to u...

This paper improves van Zyl’s Nonnegative Eigenvalue Decomposition (NNED). Orientation angle compensation and helix scattering are introduced to the decomposition. The volume scattering parameters that explain the most cross-polarized power are selected. If volume scattering and helix scattering explain all cross-polarized power in the measured coherency matrix, then simply perform van Zyl deco...

The transmembrane helix of glycophorin A contains a seven-residue motif, LIxxGVxxGVxxT, that mediates protein dimerization. Threonine is the only polar amino acid in this motif with the potential to stabilize the dimer through hydrogen-bonding interactions. Polarized Fourier transform infrared spectroscopy is used to establish a robust protocol for incorporating glycophorin A transmembrane pept...

We have recently shown that metamaterials composed of three-dimensional gold helices periodically arranged on a square lattice can be used as compact "thin-film" circular polarizers with one octave bandwidth. The physics of the motif of these artificial crystals is closely related to that of microwave sub-wavelength helical antennas in end-fire geometry. Here, we systematically study the depend...

The 97-residue M2 protein from Influenza A virus forms H+-selective ion channels which can be attributed solely to the homo-tetrameric alpha-helical transmembrane domain. Site-directed infrared dichroism spectra were obtained for the transmembrane domain of M2, reconstituted in lipid vesicles. Data analysis yielded the helix tilt angle beta=31.6(+/-6.2) degrees and the rotational pitch angle ab...

Talin is a large dimeric protein that couples integrins to cytoskeletal actin. Here, we report the structure of the C-terminal actin-binding domain of talin, the core of which is a five-helix bundle linked to a C-terminal helix responsible for dimerisation. The NMR structure of the bundle reveals a conserved surface-exposed hydrophobic patch surrounded by positively charged groups. We have mapp...

The existing producing processes of micro spiral swimmers are complex. Here, a microswimmer with magnetic layer on the surface carbon nanotubes is proposed, which has simple process. For microswimmer, its equations velocities and out-step frequency deduced. Using these equations, their changes related parameters investigated. Results show that proportional to radius helix angle nanotubes, frequ...

OBJECTIVE To determine if there is a significantly different effect on vertical changes during phase I palatal expansion treatment using a quad helix and a bonded rapid maxillary expander in growing skeletal Class I and Class II patients. MATERIALS AND METHODS This retrospective study looked at 2 treatment groups, a quad helix group and a bonded rapid maxillary expander group, before treatmen...

BACKGROUND Accurate interpretations of cardiac functions require precise structural models of the myocardium, but the latter is not available always and for all species. Although scaling or substitution of myocardial fiber information from alternate species has been used in cardiac functional modeling, the validity of such practice has not been tested. METHODS Fixed mouse (n = 10), rabbit (n ...