To characterize and compare the thymidine (TdR) and deoxycytidine (CdR) kinase isozymes of uninfected and herpesvirus-infected cells: (i) the subcellular distribution of the isozymes has been studied; (ii) a specific assay for CdR kinase has been devised; (iii) the TdR kinase isozymes have been partially purified; and (iv) the purified enzymes have been analyzed by disc polyacrylamide gel elect...

The members of the Herpesvirales containing more than 130 different herpesviruses have already been isolated from all animal species representing the higher steps of evolution. According to our knowledge, eight herpesviruses are classified as human herpesviruses: Herpes simplex virus 1 (Human herpesvirus 1, HHV-1), Herpes simplex virus 2 (Human herpesvirus 2, HHV-2), Varicella-zoster virus (Hum...

To further characterize phocid herpesvirus type 1 (PhHV-1) at the molecular level, a cluster of genes comprising the complete unique short (Us) region of PhHV-1 has been cloned and sequenced. Within this region, ORFs were detected that code for the equivalent of the Us 2- protein of herpes simplex virus (HSV), a putative protein kinase, and for the glycoprotein equivalents gG, gD, gI and gE. In...

The lifelong infection by varicelloviruses is characterized by a fine balance between the host immune response and immune evasion strategies used by these viruses. Virus-derived peptides are presented to cytotoxic T lymphocytes by major histocompatibility complex (MHC) class I molecules. The transporter associated with antigen processing (TAP) transports the peptides from the cytosol into the e...

Introduction: Infection with the human immunodeficiency virus (HIV) can result in severe immune suppression, often allowing infections that would normally be controlled by the immune system to persist in HIV-positive individuals. Many of these infections can in turn affect the dynamics of HIV disease progression. Specifically, we hypothesize that herpesvirus infections are associated with more ...

A herpesvirus was isolated from the liver of a captive-bred American kestrel (Falco sparverius) which had died of inclusion body disease. Initial isolation was achieved in chicken embryo fibroblasts after three blind passages. Cell-adapted virus produced a distinct rounding of CEF cells within 24 to 48 h. Biologic and serologic tests suggested that the kestrel virus is similar to falcon herpesv...

Herpesvirus anguillae (HVA), also known as anguillid herpesvirus 1 (AngHV1), is one of the relevant viruses in wild and cultured anguillid eels. Here, the complete genome sequence of strain HVA980811, isolated in Chiayi, Taiwan, is reported. The genotype of the eel herpesviruses in Taiwan is supposed to be identical to the Japanese AngHV1.