Abstract The method for micro-propagation of rubber ( Hevea brasiliensis Muell. Arg ) by somatic embryo has been conducted. aim the research will describe effect media used callus induction on formation plants. Embryonic had in 3 media, i.e.; MH1, MH2, and MH3. After induction, all were induced expression. Maturation cotyledons was performed using liquid with filter paper as a bridge to absorb ...

Metal cofactors are necessary for the activity of alkylation by prenyl transfer in enzyme-catalyzed reactions. Rubber transferase (RuT, a cis-prenyl transferase) associated with purified rubber particles from Hevea brasiliensis, Parthenium argentatum and Ficus elastica can use magnesium and manganese interchangably to achieve maximum velocity. We define the concentration of activator required f...

The cytosolic mevalonate (MVA) pathway in Hevea brasiliensis latex is the conventionally accepted pathway which provides isopentenyl diphosphate (IPP) for cis-polyisoprene (rubber) biosynthesis. However, the plastidic 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway may be an alternative source of IPP since its more recent discovery in plants. Quantitative RT-PCR (qRT-PCR) expression profiles ...

Rubber (cis-1,4-polyisoprene), an important raw material for many industrial uses, is synthesized in the latex of Hevea brasiliensis (Willd. ex A. Juss.) Müll. Arg. We postulated that the genes uniquely or preferentially expressed in the latex may be important for rubber biosynthesis. We constructed cDNA libraries from the latex of H. brasiliensis to investigate the genes expressed in the latex...

Latex from Hevea brasiliensis contains 30-50% (w/w) of natural rubber (cis-1,4-polyisoprene), the important raw material for many rubber industries. We have constructed a cDNA library from the latex of H. brasiliensis to investigate the expressed genes and molecular events in the latex. We analyzed 412 expressed sequence tags (ESTs). More than 90% of the EST clones showed homology to previously...

Natural rubber (Hevea brasiliensis Wild. Ex A. Juss.) Müell. Arg. is an important commodity crop grown in world over for industrial raw material latex various products, mainly tyre manufacturing. Hevea propagation through clones evolved by breeding as cultivars with desired characters. This article presented the biomass and nutrient accumulation of four viz. RRII 105, 118, 203 GT1 at 30 years a...

Rubber has been grown for several decades in Africa, notably in Ivory Coast. Although the yields obtained with selected clones are good, some problems with adaptation to edaphic and climatic conditions have yet to be solved. Of these problems, tree breakage due to violent winds during frequent storms is a major handicap. Some clones are more resistant to wind damage. However, this resistance tr...

Latex exploitation enhances latex regeneration in rubber trees. The latex exploitation-caused latex flow lasts from 10 min to a few hours, which is convenient for exploring the transcript profiling of latex metabolism-related genes at the different stages of latex flow. In the present study, the expression pattern of 62 latex metabolism-related genes involved in water transportation, carbohydra...

Abstract Natural rubber is an important industrial raw material and commercially produced by trees (Hevea brasiliensis). The sucrose transporter HbSUT3 plays essential role in production. Its expression latex (cytoplasm of rubber-producing laticifers) induced bark treatment with Ethrel, ethylene releaser, the inducing effect correlates well Ethrel-stimulated yield increase. However, mechanisms ...