نتایج جستجو برای: high cell density culture

تعداد نتایج: 3901653  

Journal: :journal of dentistry, tehran university of medical sciences 0
f. agha-hosseini m. khazabb a. parvaneroo

statement of problem: many studies have indicated that genetic disturbances are common findings in patients with oral squamous cell carcinoma (oscc). identification of these changes can be helpful in diagnostic procedures of these tumors. purpose: the aim of this study was to appraise the chromosomal disorders in blood and tissue patients with oscc. methods and materials: in this descriptive st...

Journal: :iranian journal of basic medical sciences 0
mohamadreza baghaban eslaminejad stem cell department, cell science research centre, royan institute, acecr, tehran, iran hamid nazarian stem cell department, cell science research centre, royan institute, acecr, tehran, iran fahimeh falahi stem cell department, cell science research centre, royan institute, acecr, tehran, iran leila taghiyar stem cell department, cell science research centre, royan institute, acecr, tehran, iran mohamad taghi daneshzadeh royan animal facility, karaj, iran

objective(s) mesenchymal stem cells (mscs) from large animals as goat which is genetically more closely related to human have rarely been gained attentions. the present study tried to isolate and characterize mscs from goat bone marrow. materials and methods fibroblastic cells appeared in goat marrow cell culture were expanded through several subcultures. passaged-3 cells were then differentiat...

Journal: :Biotechnology progress 2000
J W Kim T J Park D D Ryu J Y Kim

Yarrowia lipolytica is a potentially useful host for heterologous protein production. To develop an efficient culture method for high cell density cultivation and heterologous gene expression of Y. lipolytica, the effects of medium components and their concentrations on the growth of Y. lipolytica have been investigated. Addition of yeast extract to the culture media was found to significantly ...

2011
Martin Hoffmann Jens-Peer Kuska Matthias Zscharnack Markus Loeffler Joerg Galle

Therapeutic application of mesenchymal stem cells (MSC) requires their extensive in vitro expansion. MSC in culture typically grow to confluence within a few weeks. They show spindle-shaped fibroblastoid morphology and align to each other in characteristic spatial patterns at high cell density. We present an individual cell-based model (IBM) that is able to quantitatively describe the spatio-te...

Journal: :Journal of microbiology and biotechnology 2008
Jong Hyun Yoon Jong-Hwan Shin Eun Kyung Ahn Tai Hyun Park

Controlling the light energy and major nutrients is important for high cell density culture of cyanobacterial cells. The growth phase of Anabaena variabilis can be divided into an exponential growth phase and a deceleration phase. In this study, the cell growth in the deceleration phase showed a linear growth pattern. Both the period of the exponential growth phase and the average cell growth r...

Journal: :Biotechnology and bioengineering 2003
Sung Ho Yoon Mee-Jung Han Sang Yup Lee Ki Jun Jeong Jong-Shin Yoo

Combined transcriptome and proteome analysis was carried out to understand metabolic and physiological changes of Escherichia coli during the high cell density cultivation (HCDC). The expression of genes of TCA cycle enzymes, NADH dehydrogenase and ATPase, was up-regulated during the exponential fed-batch period and was down-regulated afterward. However, expression of most of the genes involved...

Journal: :Applied biochemistry and biotechnology 2010
Young-Jin Son Ji-Young Bae Seon-Ha Chong Hui Sun Lee Sang Hyun Mo Tae Yoon Kim Han Choe

Superoxide dismutase (SOD) catalyzes the dismutation of the biologically toxic superoxide anion into oxygen and hydrogen peroxide and is deployed by the immune system to kill invading microorganisms. Extracellular SOD (EC-SOD) is a copper- and zinc-containing glycoprotein found predominantly in the soluble extracellular compartment that consists of approximately 30-kDa subunits. Here, we purifi...

2003
Sung Ho Yoon Mee-Jung Han Sang Yup Lee Ki Jun Jeong Jong-Shin Yoo

Combined transcriptome and proteome analysis was carried out to understand metabolic and physiological changes of Escherichia coli during the high cell density cultivation (HCDC). The expression of genes of TCA cycle enzymes, NADH dehydrogenase and ATPase, was up-regulated during the exponential fed-batch period and was down-regulated afterward. However, expression of most of the genes involved...

Journal: :Journal of cell science 1990
B Schumpp E J Schlaeger

The purpose of the present investigation was to optimize the culture conditions in suspension of the HL-60 cell line for high-density production. The optimized HL medium was a mixture of RPMI-1640, DMEM, HamF12 and IMDM media supplemented with transferrin, insulin, Primatone RL, Pluronic F68, ethanolamine and selenite. Under these conditions, whether serum was added or not, cells grew to up to ...

2005
Michael H. Kim Vipin K. Rastogi Joseph J. DeFrank

Organophosphorus hydrolases (OPH) are of great interest to the U. S. Army for their potential use in the non-toxic, non-corrosive decontamination against VX. OPH genes had been cloned and over expressed in Escherichia coli by other researchers. However, a common problem has been that of a low expression level. In this study, batch cultures in both complex (e.g., Lauria Broth) and minimal (succi...

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