High performance liquid chromatography (HPLC) has already been essential as a powerful tool for fine separations. However, still now, some apparatuses, e.g., high pressure pumps and high performance columns, have rapidly been developed in order to establish fast chromatography techniques with high efficiency. On the other hand, the further progress of chromatographic technologies needs the purs...

In proteomic investigations, a number of different separation techniques can be applied to fractionate whole cell proteomes into more manageable fractions for subsequent analysis. In this work, utilizing HPLC and mass spectrometry for protein identification, two different fractionation methods were compared and contrasted to determine the potential of each method for the simple and reproducible...

The adsorption behaviour of two commercial preparations of human IgG onto a polystyrene latex surface was studied. The adsorption isotherms obtained differed markedly; one preparation showed a plateau value of 0.4 microgram cm-2 which was reached at 0.1 g l-1, whereas the other preparation showed no plateau value within the concentration range studied (0.1-7.0 g l-1). Characterization by means ...

Efficient fractionation of peptides is an essential prerequisite for comprehensive analysis of complex protein mixtures by shotgun mass spectrometry. The separation of peptides by isoelectric focusing is particularly attractive due to its orthogonality to reverse-phase HPLC. Here, we present a protocol for in-gel peptide isoelectric focusing using immobilized pH gradient strips. The method show...

Elevated intraocular pressure may lead to retinal ganglion cell injury and consequent visual deficits. Chronic intraocular pressure increase is a major risk factor for glaucoma, a leading blinding disease, and permanent visual deficits can also occur following acute pressure increments due to trauma, acute glaucoma or refractive surgery. How pressure affects retinal neurons is not firmly establ...

glaucoma is the second leading cause of irreversible blindness and a neurodegenerative disease with a complex pathogenesis. increasing evidence suggests that oxidative stress and mitochondrial dysfunction have crucial roles in most neurodegenerative diseases such as glaucoma. the conventional clinical treatment for glaucoma is lowering the intraocular pressure (iop). some patients have normal i...

this paper reviews recent strategies used for increasing specific yield and productivity in high cell density cultures. high cell density cultures offer an efficient means for the economical production of recombinant proteins. however, there are still some challenges associated with high cell density cultivation (hcdc) techniques. a variety of strategies in several aspects including host design...

this prospective study evaluated the long-term results of intraoperative 5-fu in glaucoma patients undergoing trabeculectomy. 14 patients categorized as high risk or medium risk underwent trabeculectomy with 5-fu and were followed for a mean period of 32 months. patients were evaluated for visual acuity, cup-disc ratio and intraocular pressure (iop); the number of medications was also taken int...

results mycoflora profiles of fresh and stored rice grains showed that aspergillus species (37.3%, 40.7%) were the predominant fungal agents, followed by fusarium (21.6%, 16.2%), mucor (19.6%, 16.7%) and rhizopus (9.8%, 11.1%), respectively. in hplc analysis, most of the rice samples (96.7%) collected were found to be positive for fb1 with mean levels ranging from not detected to 56.2 mg/kg for...

background: this study investigated blackberry (persian mulberry) effects on apo a-i, apo b, high-sensitivity-c-reactive protein (hs-crp), and systolic blood pressure (sbp) and diastolic blood pressure (dbp) in dyslipidemic patients. materials and methods: in this 8-week randomized clinical trial, 72 dyslipidemic patients were randomly divided into two groups: intervention (300 ml/day blackberr...