Abstract Adenylyl cyclase (AC) is an enzyme that catalyses the formation of second messenger molecule, 3′,5′-cyclic adenosine monophosphate (cAMP) from 5′-adenosine triphosphate (ATP). cAMP, in turn, regulates key physiological processes such as cell division, growth, reproduction, development and response to stress. However, while cAMP increasingly becoming important signalling molecule higher...

Introduction: Maintenance of neurogenesis depends on the function of some histone-modifying enzymes; including Enhancer of zeste homolog 2 (EZH2) and histone acetyltransferases (P300). The mechanism of epigenetic regulation and gene expression underlying the transition of human umbilical cord blood mesenchymal stem cells (hUCB-MSCs) into MNs has not been fully clarified.  Methods: Two morphoge...

Binge drinking is common during adolescence and can lead to the development of psychiatric disorders, including alcoholism in adulthood. Here, the role and persistent effects of histone modifications during adolescent intermittent ethanol (AIE) exposure in the development of anxiety and alcoholism in adulthood were investigated. Rats received intermittent ethanol exposure during post-natal days...

Mating-type silencing in Schizosaccharomyces pombe is brought about by cooperative interactions between cis-acting DNA sequences flanking mat2P and mat3M and the trans-acting factors, namely Swi6, Clr1-Clr4, Clr6, and Rik1. In addition, DNA repair gene rhp6, which plays a role in post-replication DNA repair and ubiquitination of proteins including histones, is also involved in silencing, albeit...

Prokaryotic cells synthesize a set of small, usually basic proteins that bind DNA and are collectively called histonelike proteins because their biochemical properties resemble eukaryotic histones. Whereas the eukaryotic histones have a well defined function in packaging DNA through restraining left-handed toroidal DNA supercoils in nucleosomes, the functions of the prokaryotic histone-like pro...

In this study the effect of a methylated derivative of nitrogen mustard (mechloroethamin) on solubilized and reconstituted chromatin was investigated using UVNis spectroscopy, DNA and SDS gel electrophoresis and densitomeoic scanning techniques, The results indicated that the 210 and 260 nm absorbances of reconstituted chromatin were decreased in a dose-dependent manner after treatment wit...

Highly effective yet chemoselective chemical transformation strategies enable the facile access and precise modification of complicated biomacromolecules. In particular, application desulfurization chemistry expands dimension protein synthesis with cysteine-based peptide ligation. Considering existing methods, a milder, faster, easier strategy is still required for increasing complexity protein...

Progress in protein labeling by gene fusion with fluorescent protein-encoding genes and microscopic apparatuses, such as a confocal laser-scanning microscope, has enabled time-lapse analysis of meiotic chromosome movement living plant cells. Here, we constructed the pAtDMC1:H2B:GFP introduced it into Arabidopsis thaliana. Whole-genome sequencing confirmed insertion locus between AT3G14830 AT3G1...