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As larger-scale cloning projects become more prevalent, there is an increasing need for comparisons among high fidelity DNA polymerases used for PCR amplification. All polymerases marketed for PCR applications are tested for fidelity properties (i.e., error rate determination) by vendors, and numerous literature reports have addressed PCR enzyme fidelity. Nonetheless, it is often difficult to m...
Introduction A thermoacoustic engine is a device absorbing heat at the hot heat exchanger and releasing heat at the cold heat exchanger while producing acoustic work as an output. Engines are built to carry a load, which in the case of an acoustic device, might include a thermoacoustic refrigerator [4], a (possibly linear) electric generator [2] etc. Coupling between the engine and the load inf...
The PCR technique often yields nonspecific products. To overcome this problem, a simple, specific and efficient method was designed: touch-up and loop incorporated primers (TULIPS)-PCR. This approach utilizes loop primers (i.e., additional nontemplate 5' sequence that self-anneals to the 3' region and inhibits initiation of polymerization). Upon heating of the reaction, the primers melt, initia...
The role of proliferation in the development of 19S antibody-forming cells in the primary response has been investigated in an in vitro system. Spleen cell suspensions from normal, unimmunized mice were cultured in vitro in the presence of mammalian erythrocytes and the number of 19S hemolytic plaque-forming cells that arose 4 days later was measured. The hot pulse technique for the selective i...
The Papua New Guinea (PNG) tsunami of 1998 is re-examined through a detailed review of the field survey as well as numerous numerical computations. The discussion of the field survey explores a number of possible misinterpretations of the recorded data. The survey data are then employed by a numerical model as a validation tool. A Boussinesq model and a nonlinear shallow water wave (NLSW) model...
objective(s): use of a positron emission tomography (pet)/single-photonemission computed tomography (spect) system facilitates the simultaneousacquisition of images with fluorine-18 fluorodeoxyglucose (18f-fdg) andtechnetium (99mtc)-tetrofosmin. however, 18f has a short half-life, and 511kev compton-scattered photons are detected in the 99mtc energy window.therefore, in this study, we aimed to ...
A comprehensive study on the effects of photochemical aging exhaust emissions from a vehicle equipped with gasoline direct injection engine when operated over seven different driving cycles was assessed using an oxidation flow reactor. Both primary and secondary aerosol production were measured Federal Test Procedure (FTP), LA92, New European Driving Cycle (NEDC), US06, Highway Fuel Economy (HW...
We transposed Dissociation (Ds) elements from three start loci on chromosome 5 in Arabidopsis (Nossen ecotype) by using a local transposition system. We determined partial genomic sequences flanking the Ds elements and mapped the elements' insertion sites in 1,173 transposed lines by comparison with the published genomic sequence. Most of the lines contained a single copy of the Ds element. One...
There is nothing quite so helpless in this world as a baby broiler chick which has been snatched from the warmth of its surrogated mother, carted umpteen kilometers in a box dumped unceremoniously in a broiler shed and then told to get on with the job of making the boss a wealthy man. Yet within the space of 14 days, that same chick, under optimum conditions, will have increased its day-old bod...
Directly detecting thermal emission from young extrasolar planets allows measurement of their atmospheric compositions and luminosities, which are influenced by their formation mechanisms. Using the Gemini Planet Imager, we discovered a planet orbiting the ~20-million-year-old star 51 Eridani at a projected separation of 13 astronomical units. Near-infrared observations show a spectrum with str...
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