BACKGROUND Value of serum ferritin (SF) as an iron store index in hemodialysis (HD) patients has been questioned, especially at ranges >or=200. The objective of this study was to determine the variability of SF in patients with high SF (500-1,200.) and low TSAT (<or=25%). METHODS This was a multicenter observational study. Data were obtained from Dialysis Patients' Response to IV Iron with El...

Ferritin, the iron-storing molecule, is made by the assembly of various proportions of 2 different H and L subunits into a 24-mer protein shell. These heteropolymers have distinct physicochemical properties, owing to the ferroxidase activity of the H subunit, which is necessary for iron uptake by the ferritin molecule, and the ability of the L subunit to facilitate iron core formation inside th...

Mitochondrial function depends on iron-containing enzymes and proteins, whose maturation requires available iron for biosynthesis of iron-sulfur clusters and heme. Little is known about how mitochondrial iron homeostasis is maintained, although the recent discovery of a mitochondrial ferritin in mammals and plants has uncovered a potential key player in the process. Here, we show that Drosophil...

Eleven commercial kits for serum ferritin have been compared. Seven kits used RIA and four IRMA methodology. Seven kits used human liver ferritin, two human spleen ferritin and two undefined human ferritin as standard material. Incubation times varied between 30 minutes and 36 hours. Only four kits gave normal ranges determined with the kit in question. All values were compared with the kit in ...

Low density lipoprotein (LDL) and epidermal growth factor (EGF) bind to receptors on the surface of human fibroblasts and are internalized in coated vesicles. Each of the ligands has been studied separately by electron microscopy in human fibroblasts using ferritin-LDL as one visual probe and 125I-EGF as a second visual probe. A mutant strain of human fibroblasts (J.D.) has been described in wh...

Ferritin is the main iron storage protein that plays an important role in homeostasis and concerned with many physiological functions pathologic disorders. Clinically, ferritin mainly used as a biomarker for total body stores. Serum has critical function both deficiency overload related to diagnosis treatment. High levels of serum tissue are coronary artery disorder, cancer, bad results after s...

Ferritin plays a key role in determining the intracellular fate of iron and is highly regulated by the iron status of the cell. We have identified a cis-acting element in the transcribed but nontranslated 5' leader sequence of human ferritin heavy-chain mRNA. In transiently transfected murine fibroblasts, the presence of a 157-nucleotide region of the 5' leader sequence was found to be necessar...

In the present study, we show that hydroxyurea-inactivated ribonucleotide reductase protein M2 has a destabilized iron center, which readily releases iron. In addition, evidence is presented which indicates that single or multistep selection for hydroxyurea resistance, in a variety of mammalian cell lines, leads to alterations in the expression of the gene for the iron storage protein, ferritin...