Synthetic peptides selected from HLA-DQ and HLA-DP glycoproteins were coupled to Sepharose, and used for the isolation of anti-HLA Class II antibodies from the immune sera of rabbits immunized with human lymphoblastoid cells expressing Class II antigens. Antibodies from early and late bleedings displayed remarkable differences in affinity for peptides and for soluable membrane proteins: these d...

Background Patients can form antibodies to foreign human leukocyte antigen (HLA) Class I antigens after exposure allogeneic cells. These anti-HLA class bind transfused platelets (PLTs) and mediate their destruction, thus leading PLT refractoriness. with refractoriness need HLA-matched PLTs, which require expensive HLA typing of donors, antibody analyses patient sera and/or crossmatching. An alt...

PURPOSE OF REVIEW This review addresses the concept that human leukocyte antigen (HLA) antibody specificity should be determined to HLA epitopes rather than HLA antigens. RECENT FINDINGS HLAMatchmaker is a computer algorithm that considers small configurations of polymorphic residues referred to as eplets as essential components of HLA epitopes. This overview describes recent developments tha...

The effect of recombinant human interferon gamma (IFN-gamma) on the induction of HLA class II (HLA-DR, -DP, -DQ) antigen expression on human corneal epithelial (HCE) cells was examined in different stages of culture. Primary cultures were established with limbal explants without endothelium. HCE cells in Stage 1 and Stage 2, with cells negative and positive for the 64K corneal keratin (the mark...

The genomic organization and number of human HLA-DRB class II genes differs between haplotypes in the human population. In contrast, both HLA-DQ and -DP class II subregions are conserved. Understanding of the evolutionary relationship and age of HLA-DR haplotypes has been obtained by nucleotide sequence determination and phylogenetic analyses of intron sequences of all known DRB genes from diff...

Background The clinical and economic impact of severe drug hypersensitivity is huge for both healthcare and pharmaceutical industry; however, no method for predicting culprit candidate t drugs under clinical development exists currently. As genetic variants in human-leukocyte antigens (HLA) have been linked to inter-individual differences in the risk of drug hypersensitivity, we ascertained whe...

HLAMatchmaker is a computer algorithm that assesses human leukocyte antigen (HLA) compatibility at the structural level by intralocus and interlocus comparisons of polymorphic amino acid triplets in antibody-accessible sequences of HLA class I molecules. This program permits the identification of mismatched HLA antigens that share all of their polymorphic triplets with the patient's HLA antigen...

background: human leukocyte antigen-e (hla-e)is a non-classical major histocompatibility complex (mhc) class i antigens which expressed on extra villous cytotrophoblast, which interacts with nkg2a, is an inhibitory receptor on natural killer (nk) cells and leading to down regulation of immune response in the maternal-fetal interface and provides maternal immune tolerance of the fetus. objective...

In this report, data are presented on the regulation of MHC class II antigen expression by a mediator present in supernatants of human mixed leukocyte cultures (MLC-SN), and which is different from IFN-gamma. The capacity of supernatants to induce antigen expression did not correspond to titers of IFN-gamma. Removal of IFN-gamma using either dialysis against pH 2 or neutralizing mAb against hum...

Human leukocyte antigen (HLA) Class I and Class II antigens can be genetically extremely diverse, play a central role in immunity and are of primary importance in several branches of medicine. By presenting fragments of pathogens or altered ‘self’ proteins, HLA molecules activate T lymphocytes and are essential in adaptive immune responses against infection or cancer. Clinically, certain HLA ty...