BACKGROUND The spermatogonial stem cell (SSC) pool in the testes of non-human primates is poorly defined. METHODS To begin characterizing SSCs in rhesus macaque testes, we employed fluorescence-activated cell sorting (FACS), a xenotransplant bioassay and immunohistochemical methods and correlated our findings with classical descriptions of germ cell nuclear morphology (i.e. A(dark) and A(pale...

An economical and simplified procedure to derive and propagate fully functional lines of undifferentiated rat spermatogonia in vitro is presented. The procedure is based on the formulation of a new spermatogonial culture medium termed SG medium. The SG medium is composed of a 1:1 mixture of Dulbecco modified Eagle medium:Ham F12 nutrient, 20 ng/ml of GDNF, 25 ng/ml of FGF2, 100 microM 2-mercapt...

Spermatogonial stem cells (SSCs) also known as germ stem cells (GSCs) are the basis of spermatogenesis process in the testis. Furthermore, they are also valuable cells with different applications in developmental biology, transgenesis technology, and clinic. Understanding the new findings related to the cell and molecular biology of SSCs and the methods for isolation and maintenance of these ce...

background: creation of artificial gametes may provide a universal solution for these patients of lacking gametes. stem cell technology may provide a way to obtain fully functional gametes. retinoic acid (ra) can initiate meiosis. several studies have demonstrated that ra can promote sperm cells differentiation from mouse embryonic stem cells (mescs) and other cells from human embryonic stem ce...

introduction: the spermatogonial stem cells (sscs) form the basis of spermatogenesis. in vitro culture of germ cells is useful for the purification and increasing of sscs for transplantation. in the present study, we examined: in vitro the effects of co-culture with sertoli cells, gdnf, scf and gm-csf on efficiency of spermatogonial cells colony formation and spermatogenesis induction in the re...

Although many environmental agents are established male germ cell mutagens, few are known to induce mutations in spermatogonial stem cells. Stem cell mutations are of great concern because they result in a permanent increase in the number of mutations carried in sperm. We investigated mutation induction during mouse spermatogenesis following exposure to benzo(a)pyrene (BaP). MutaMouse males wer...

Background: Proliferation of spermatogonial stem cells (SSCs) can be a treatment for infertile men. Here, we design an efficient method based on culturing in the presence of Sertoli cells to improve the expression level of some specific spermatogonia stem cell genes during two weeks post culture. Materials and Methods: Cells were derived from neonatal (2-6 days old) mice testes and were cultur...

background: in most mammals, the testis is always maintained at a lower temperature than that in the abdomen, and exposure of the testis to body temperature causes degeneration of germ cells. objective: in this research, the long effect of heat exposure on sperm parameters and microstructure of mouse testis were investigated. cryptorchid mouse were induced by exposure to abdominal heat. materia...

Although spermatogenesis is essential for reproduction, little is known about spermatogonial stem cells. These cells provide the basis for spermatogenesis throughout adult life by undergoing self-renewal and by providing progeny that differentiate into spermatozoa. A major impediment to our understanding of the biology of these stem cells is the inability to distinguish them from spermatogonia ...

The maintenance of cycling cell lineages relies on undifferentiated subpopulations consisting of stem and progenitor pools. Features that delineate these cell types are undefined for many lineages, including spermatogenesis, which is supported by an undifferentiated spermatogonial population. Here, we generated a transgenic mouse line in which spermatogonial stem cells are marked by expression ...