Human induced pluripotent stem cells (hiPSCs) have demonstrated great potential for differentiation into diverse tissues. We report a straightforward and highly efficient method for the generation of iPSCs from PBMCs. By plating the cells serially to a newly coated plate by centrifugation, this protocol provides multiple healthy iPSC colonies even from a small number of PBMCs. The generated iPS...

Full-term human umbilical cord contains three blood vessels: two arteries coiled around a vein and surrounded by Wharton's jelly, a mucous tissue with few mesenchymal stromal cells and abundant extracellular matrix. Umbilical vessels lack innervations, thus endothelial cells must play a role in the control of blood flow. The aim of this study was to investigate in human umbilical cord the expre...

The term “cord blood” is used for blood that is drawn from the umbilical cord and the placenta after a baby is born. Up until recently this afterbirth was discarded as medical waste. Cord blood contains stem cells that may be frozen for later use in medical therapies, such as stem cell transplantation or regenerative medicine. The umbilical cord and placenta are rich sources of stem cells. Thes...

PURPOSE OF REVIEW To review the available clinical and biological advances of umbilical cord blood allogeneic stem cell transplantation in pediatric and adult patients. RECENT FINDINGS Recent large international studies suggested that allogeneic umbilical cord blood transplantation may potentially emerge as the frontline stem cell source for pediatric patients with hematopoietic malignancies ...

Both human and mouse c-kit ligand induced differentiation of human mast cells in a long-term culture of the mononuclear cells of umbilical cord blood. Growth factor activity for human mast cells present in conditioned medium of BALB/3T3 fibroblasts was due to mouse c-kit ligand. Recombinant c-kit ligand induced differentiation and proliferation of mast cell progenitors in early stages of cultur...

Objective(s):Immunosuppressive property of mesenchymal stem cells (MSCs) has great attraction in regenerative medicine especially when dealing with tissue damage involving immune reactions. The most attractive tissue sources of human MSCs used in clinical applications are bone marrow (BM), adipose tissue (AT), and Wharton's jelly (WJ) of human umbilical cord. The current study has compared immu...

objective: receptor activator of nuclear factor-kappa b ligand (rankl) appears to be an osteoclast-activating factor, bearing an important role in the pathogenesis of multiple myeloma. some studies demonstrated that u-266 myeloma cell line and primary myeloma cells expressed rank and rankl. it had been reported that the expression of myeloid and monocytoid markers was increased by co-culturing ...

and Laboratory of Regenerative Medicine, Department of Medical Research, and Department of Surgery, E-Da Hospital, I-Shou University, Kaohsiung, Taiwan. Received: June 24, 2015 Accepted: March 16, 2016 Address reprint request and correspondence to: Ying-Hsien Kao, Laboratory of Regenerative Medicine, Department of Medical Research, and Po-Huang Lee, Department of Surgery, E-Da Hospital, No. 1, ...