In a step towards a tetravalent dengue virus subunit vaccine which is economical to produce, highly immunogenic and stable, a hybrid dengue virus envelope (E) protein molecule has been constructed. It consists of 36 amino acids from the membrane protein, the N-terminal 288 amino acids of the dengue-2 virus E protein plus amino acids 289-424 of the dengue-3 virus E protein. It has been engineere...

We have developed a bacterial "two-hybrid" system that readily allows selection from libraries larger than 10(8) in size. Our bacterial system may be used to study either protein-DNA or protein-protein interactions, and it offers a number of potentially significant advantages over existing yeast-based one-hybrid and two-hybrid methods. We tested our system by selecting zinc finger variants (fro...

Computational analysis, design and expression of hybrid antibody in Single Chain Fragment Variable (scFv) form for identifying surface antigen factor H binding protein (fHbp) Neisseria meningitidis

Genetic high throughput screens have yielded large sets of potential protein-protein interactions now to be verified and further investigated. Here we present a simple assay to directly visualize protein-protein interactions in single living cells. Using a modified lac repressor system, we tethered a fluorescent bait at a chromosomal lac operator array and assayed for co-localization of fluores...

A large number of nuclear-encoded proteins are imported into chloroplasts after they are translated in the cytosol. Import is mediated by transit peptides (TPs) at the N termini of these proteins. TPs contain many small motifs, each of which is critical for a specific step in the process of chloroplast protein import; however, it remains unknown how these motifs are organized to give rise to TP...

We present a simple model for the underlying structure of protein-protein pairwise interaction graphs that is based on the way in which proteins attach to each other in experiments such as yeast two-hybrid assays. We show that data on the interactions of human proteins lend support to this model. The frequency of the number of connections per protein under this model does not follow a power law...

Eric Deeds et al. examine the statistical properties of protein– protein interaction networks and propose a physical model based on the fundamental principle of desolvation interactions between proteins. The authors examined the network of protein–protein interactions reported by the yeast two-hybrid system. They demonstrated that two published independent measurements of these interactions pro...

New genetic constructs encoding synthesis of hybrid proteins possessing organophosphorus hydrolase activity and properties of the pH sensitive analog of the green fluorescent protein were developed. It was established that 0.1 mM of the biosynthesis inducer and cultivation for 10 h after the induction were necessary for the maximum yield of the hybrid proteins in the soluble and active form in ...