BACKGROUND Pichia stipitis xylose reductase (Ps-XR) has been used to design Saccharomyces cerevisiae strains that are able to ferment xylose. One example is the industrial S. cerevisiae xylose-consuming strain TMB3400, which was constructed by expression of P. stipitis xylose reductase and xylitol dehydrogenase and overexpression of endogenous xylulose kinase in the industrial S. cerevisiae str...

The discovery of huge petroleum reserves in the 19th century was a golden opportunity that promoted industrial revolution, by offering wide variety cheap raw materials and reliable source energy. This caused an unprecedented increase commodity production specialty chemicals order to fulfill needs global markets. However, as warming environmental issues has become hazardous worried situation, re...

BACKGROUND Current biomass pretreatment by hydrothermal treatment (including acid hydrolysis, steam explosion, and high-temperature steaming) and ionic liquids generally generate inhibitors to the following fermentation process. Furfural is one of the typical inhibitors generated in hydrothermal treatment of biomass. Furfural could inhibit cell growth rate and decrease biofuel productivity of m...

Abstract As a result of the global demand for sustainable products, suitable alternative to resorcinol-formaldehyde aerogels, which are frequently used as precursors carbon is searched for. In this study, replacement petroleum-derived formaldehyde with natural, biobased crosslinker, namely 5-(hydroxymethyl)furfural (5-HMF) shown, and synthesis renewable, monolithic tannin aerogels demonstrated....

BACKGROUND Furfural is a major growth inhibitor in lignocellulosic hydrolysates and improving furfural tolerance of microorganisms is critical for rapid and efficient fermentation of lignocellulosic biomass. In this study, we used the RNAi-Assisted Genome Evolution (RAGE) method to select for furfural resistant mutants of Saccharomyces cerevisiae, and identified a new determinant of furfural to...

Low concentrations of furfural are formed as a side product during the dilute acid hydrolysis of hemicellulose. Growth is inhibited by exposure to furfural but resumes after the complete reduction of furfural to the less toxic furfuryl alcohol. Growth-based selection was used to isolate a furfural-resistant mutant of ethanologenic Escherichia coli LY180, designated strain EMFR9. Based on mRNA e...