An efficient protocol of common bean transformation using Agrobacterium tumefaciens is still unavailable. Several regeneration reports are available using cotyledonary node or embryonic axis as the starting explants. These explants were used for direct organogenesis exploiting the meristems for direct shoot production. Attempts were made to transform cotyledonary node explants and embryonic axe...

Ferrochelatase catalyzes the insertion of Fe2+ into protoporphyrin IX to generate protoheme. A putative mature region of a cucumber ferrochelatase cDNA (hemH) was overexpressed in Escherichia coli and purified to homogeneity (40 kDa). The optimum pH was 7.7, and the apparent K(m) values for deuteroporphyrin IX and Fe2+ were 14.4 microM and 4.7 microM, respectively. The activity of the ferrochel...

The DREB (dehydration-responsive element binding)-type transcription factors are classified into six subgroups, named A-1 to A-6. The members of DREB A-1 and A-2 subgroups have been reported to be involved in response to various abiotic stresses. However, there were only a few genes belonging to A-3 to A-6 subgroups to be reported. In this study, we cloned a DREB A-4 subgroup gene from maize (Z...

PURPOSE To determine whether resurfacing submacular human Bruch's membrane with a cell-deposited extracellular matrix (ECM) improves retinal pigment epithelial (RPE) survival. METHODS Bovine corneal endothelial (BCE) cells were seeded onto the inner collagenous layer of submacular Bruch's membrane explants of human donor eyes to allow ECM deposition. Control explants from fellow eyes were cul...

Explants of leaves, petals, anthers, styles and ovaries of C. speciosus, C. cancellatus, C. gilanicus and C. almehensis were cultured on LS media with three different combinations of NAA and BAP in the dark. Callus and neoformant organs (leaf and corm-like structure and ordinary and contractileroots) were fomd on some of these explants. Corm explants from these species were also cultured on...

The Tungkal Composite of liberica coffee rejuvenation program at Jambi Province is urgently needed due to the latest status plants are old and showing diseased attacked. To support rejuvenation, provision seedlings could be done by tissue culture approach through somatic embryogenesis. This research aimed study effect 2,4-D, BA TDZ on callus formation embryo induction from leaf explants. Explan...

Regeneration of explants plays a significant role in plant transformation. Explant type, hormonal concentration, and pre-culturing period are important in transformation efficiency. To get an efficient transformation of canola and optimize regeneration conditions, different explants along with different culture media were studied. Four canola varieties were used to evaluate regeneration ability...

Microgreens growing under electric (artificial) lighting in controlled environments often have short hypocotyls, which can be difficult for machine harvest. To investigate whether early-stage dark treatment promote hypocotyl elongation without compromising microgreen yield and quality, two different seed-size species, sunflower (Helianthus annuus ‘Black oil’) arugula (Eruca sativa ‘Rocket’), we...

It has been proposed that spacing between cellulose microfibrils within plant cell walls may be an important determinant of their mechanical properties. A consequence of this hypothesis is that the water content of cell walls may alter their extensibility and that low water potentials may directly reduce growth rates by reducing cell wall spacing. This paper describes a number of experiments in...