There are a number of applications of molecular markers in agriculture such as assessing genetic diversity, generating DNA fingerprints and developing markers linked to a trait of interest, etc. Arbitrarily Primed-Polymerase Chain Reaction (AP-PCR) in which template DNA is amplified using single arbitrary primers of 10 base in length is a widely used technique. The objectives of this investigat...

Bacterial 16S ribosomal DNA (rDNA) amplicons have been widely used in the classification of uncultured bacteria inhabiting environmental niches. Primers targeting conservative regions of the rDNAs are used to generate amplicons of variant regions that are informative in taxonomic assignment. One problem is that the percentage coverage and application scope of the primers used in previous studie...

Premise of the study To investigate a more detailed evolutionary history of Lindera obtusiloba (Lauraceae) and other Lindera species, polymorphic low-copy nuclear primers were developed. Methods and Results Unigenes of the L. obtusiloba transcriptome greater than 800 bp in length were randomly chosen for initial design of 168 primers. Agarose gel electrophoresis and Sanger sequencing were use...

Objective: The aim of this study was to estimate genetic variability and phonetic relationships of 50 isolates of P. sojae from Iran. Methods: During 2005–2007, 142 isolates of Phytophthora sojae were collected from soil samples and diseased soybean plants from Lorestan, Mazandaran, Golestan and Ardabil provinces. Races of the isolates were determined. DNA of P. sojae...

Economical procedures for mapping large numbers of cosmids and YACs, using panels of somatic cell hybrids are of practical importance in genome mapping. We developed a method based on direct PCR of individual cosmid colonies with primers complementary to the cosmid vector and 2 primers complementary to the extreme 5 prime and 3 prime parts of the Alu consensus sequence (1). We also applied this...

Background: Species identification in commercially processed food and feed products is one of the important issues. This study was conducted to develop a genetic method for the detection of pig and cattle species in processed food and feed products using newly designed species-specific primers targeting mitochondrial 12S rRNA gene fragments. Methods: Two sets of specific primers were designed ...

to investigate the genetic diversity of 21 rapeseeds (brassica napus l.), 20 semi-random isj and ssr primers were used. primers produced a total number of 158 (81.5%) polymorphic bands (51 ssr and 107 isj bands). average numbers of polymorphic bands for isj, ssr and combined of them were 10.7, 5.1 and 7.9, respectively. pic values ranged from 0.0150 for specific primer o110d08 to 0.3420 for sem...

The PCR-amplification of unknown homologous or paralogous genes generally relies on PCR primers predicted from multi sequence alignments. But increasing sequence divergence can induce the need to use degenerate primers which entails the problem of testing the characteristics, unwanted interactions and potential mispriming of degenerate primers. Here I introduce easyPAC, a new software for the p...