BACKGROUND Cryptosporidium parvum causes an opportunistic infection in AIDS patients, and no effective treatments are yet available. This parasite possesses a single fatty acyl-CoA binding protein (CpACBP1) that is localized to the unique parasitophorous vacuole membrane (PVM). The major goal of this study was to identify inhibitors from known drugs against CpACBP1 as potential new anti-Cryptos...

BACKGROUND: Canine parvovirus (CPV) infection is one ofthe most common causes of infectious gastroenteritis in dogs andis a highly contagious, often fatal disease. The original virus(CPV type 2) has had some mutations since its emergence andnew variants (CPV-2a, 2b and 2c) have been reported from manycountries all around the world. Early diagnosis and treatment canprofoundly affect the disease ...

The use of reverse transcription (RT) PCR for relative quantitation of gene transcripts relies on the reproducibility of the individual RT, PCR and product measurement steps. Semi-competitive RT-PCR (RT-cPCR) uses an internal competitor template in the PCR step to improve quantitation. We have surveyed the reproducibility of RT, PCR, RT-cPCR and measurement, amplifying the glyceraldehyde-3-phos...

during 2007-2008 growing season, a total of 520 sunflower leaf samples with symptoms like vein clearing, cholorotic and necrotic local lesion, deformation and stunting which were suspected of being infected with tobacco streak virus (tsv) were collected from sunflower fields of azarbayijan-gharbi, isfahan, qom, markazi, tehran and hamedan provinces. infection with tsv in collected samples were ...

Objectives We investigated the relationship between the expression of tumor necrosis factor-inducible gene 6 (TSG-6) with inflammation and integrity of the bladder epithelium in the bladder tissues of patients with bladder pain syndrome/interstitial cystitis (BPS/IC) and the mechanism of action using a rat model of BPS/IC. Materials and Methods Expression of TSG-6 and uroplakin III was determ...

Plum pox virus (PPV) is the causal agent of sharka disease of stone fruit trees which is considered to be one of the most serious plant diseases. Sensitive techniques are needed for its diagnosis. Immunosorbent assays based on the use of monoclonal antibodies (1) and different PCR techniques have been described with or without previous immunocapture (2,3). In all instances plant extracts are ne...

background: canine parvovirus (cpv) infection is one ofthe most common causes of infectious gastroenteritis in dogs andis a highly contagious, often fatal disease. the original virus(cpv type 2) has had some mutations since its emergence andnew variants (cpv-2a, 2b and 2c) have been reported from manycountries all around the world. early diagnosis and treatment canprofoundly affect the disease ...

ObjectiveTo screen pregnant women at risk of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection during delivery using reverse-transcription polymerase chain reaction (RT-PCR) test and serum immunoglobulin (Ig) testing.MethodBetween March 31 st August 2020, consecutive admitted for labor in a single hospital were screened SARS-CoV-2 with nasopharyngeal RT-PCR swab tests detec...

Serology is based on the use of antibodies to detect circulating antibodies (immunoglobulin) or antigens. Largely used in the diagnosis of infectious diseases, it is now often replaced by DNA or RNA detection of infectious agents by molecular biology techniques such as polymerase chain reaction (PCR, rt-PCR). However, antibody detection can have some advantages on PCR techniques and numerous di...