Recombinant DNA technology is a common procedure to produce allergenic proteins in vitro. To date, more than 40 food allergens from fruits, vegetables, nuts, milk and seafood have been expressed as recombinant proteins. Since recombinant proteins can be produced in large quantities and in controlled quality, they promise to be suitable tools for precise diagnostics and research applications. "H...

BACKGROUND Downy mildews are a group of microorganisms belonging to the Chromista kingdom that can infect specific plants. When growing on plant tissues these microbes can elicit the expression of pathogenesis-related proteins (PRs), a group of stress-induced proteins frequently described as allergens in many plant species. Our aim was to verify by a proteomic approach whether the allergic reac...

Hydrolysed wheat protein (HWP), which has high emulsifying property and water retentivity, is added to various foods and cosmetics to improve their texture. Recently, several studies showed that a specific HWP formula could induce IgE-mediated hypersensitivity by skin contact and/or food ingestion. In Japan, a number of HWP-caused systemic wheat allergy cases were reported, including wheat-depe...

oral allergy syndrome (oas) is occasionally observed following consumption  of raw fruits  in  allergic adults. since this  phenomenon  was  commonly  reported  in  khorasan province of iran; we intended to check if common  diagnostic tests could be applied for differential diagnosis of oas to grapes. ige reactivity of 84 patients with oas to grape and 34 patients with oas to other fruits were ...

The IgE (immunoglobulin E) receptor of cultured rat basophilic leukemia (RBL-1) cells, shown previously to be an M, = 45,000 to 50,000 glycoprotein, was purified by repetitive affinity chromatography on IgE-Sepharose. RBL-1 cells were either labeled exogenously with “‘1 by the lactoperoxidase method or endogenously by growing cells in the presence of o-[14C]glucosamine and L-[3H]leucine. Recept...

Allergies to certain fruits such as banana, avocado, chestnut and kiwi are described in 30-70% of latex-allergic patients. This association is attributed to the cross-reactivity between the major latex allergen hevein and hevein-like domains (HLDs) from fruit class I chitinases. We aimed to assess the extent of cross-reactivity between hevein and HLDs using sera from latex-allergic patients wit...

The aim of this study was to identify the major allergens of wildflower honey in local patients with atopic disease. SDS-PAGE revealed ten protein bands of 25 to 110 kDa, with a heavy cluster in region of 40-75 kDa. Immunoblotting demonstrated seven IgE-binding bands of 39 to 110 kDa. The 60 kDa protein had the highest frequency of IgE-binding (100%) followed by 54 kDa protein (95%), thus ident...

Roasting is known to affect the protein profile and allergenicity of hazelnuts (Corylus avellana cv TGL). The aim study was investigate whether roasting techniques based on different heat transfer methods (hot air infrared), differently solubility IgE-binding capacities both soluble insoluble hazelnut fractions. immune-reactivity Cor a 9, 11 14 allergens resulted be stable after at 140 °C, for ...

Specific IgE to proteins from Tribolium confusum (TC), a flour beetle, was detected in 9/125 sera of subjects exposed to rye and wheat flour. TC RAST was not inhibited by Dermatophagoides pteronyssinus, rye or wheat flour. Immunoblot experiments showed specific binding to three proteins from adult TC or pupae, not present in rye or wheat flour. These findings suggest that TC might act as an occ...

An IgM mAb (BB10) was produced by immunization of mice with human eosinophils purified according to their abnormal low density ("hypodense" cells), and previously shown to exhibit increased IgE-dependent antiparasite cytotoxicity. This BB10 antibody, selected for positive fluorescence staining of hypodense blood or lung eosinophils and low or negative staining of normodense eosinophils or neutr...