Background Ghezel sheep are highly prolific and one of the local sheep breeds in Iran and Turkey. Growth differentiation factor-9 (GDF9) gene has been found to be essential for growth and differentiation of early ovarian follicles. Novel mutations in GDF9 have been associated with increased ovulation rates and high litter sizes in heterozygous carriers. Therefore, fecundity gene for GDF9 (FecGH...

Background: VP4 protein is as spikes on rotavirus outer capsid shell which is responsible for virus attachment to the host. VP4 induces production of neutralizing antibodies which could be used for serotyping of different isolates. Methods: Simian rotavirus SA11 gene 4 cDNA was cloned into a cloning plasmid pDONRTM by recombination reaction using clonase II enzyme mix. The resulting clone was c...

Although large numbers of temperate phages are known to exist in nature, only a small number of these have been examined in much detail. Foremost among these is the coliphage ~, which has been studied in great depth over the past two decades. However, it is already quite clear that phage ~ represents only one alternative among many with respect to any of its interesting properties. Most other t...

We have previously shown that angiotensin II (Ang II) increases the expression of the gene encoding adipocyte fatty acid synthase (FAS). Here we investigate the mechanism responsible for increased FAS gene transcription by Ang II. We demonstrate that Ang II increased luciferase activity by 3-fold in 3T3-L1 adipocytes transfected with fusion constructs linking the FAS promoter to the luciferase ...

African American women suffer higher incidence and mortality of triple negative breast cancer (TNBC) than Caucasian women. TNBC is very aggressive, causing the worst clinical outcome. We previously demonstrated that tumors from these patients express high IGF-II and exhibit high activation of the IGF signaling pathways. IGF-II gene expression is imprinted (monoallelic), promotes tumor progressi...

Zinc as an essential trace element was reported to be involved in regulation of the growth and aging of cells. In this study, rat adipose-derived mesenchymal stem cells were exposed to extremely low frequency electromagnetic field (ELF-EMF) of 50 Hz and 20 mT to evaluate whether exposure to ELF-EMF in the presence of zinc sulfate (ZnSO4) affects the telomerase reverse transcriptase (...

14 Genetic and biochemical studies have identified RNA polymerase II (Pol II) activity mutants that alter 15 catalysis, which we use as tools to investigate transcription mechanisms. Gene expression 16 consequences due to altered Pol II activity in vivo are complex. We find that alteration of Pol II catalysis 17 decreases Pol II occupancy and reporter gene expression in vivo. Both reduced and i...

zebra fish were exposed to environmentally relevant concentration of pesticides and metals fora period of 14 days. the individual and the combined toxicity of pesticides and metals were studied. damage caused to the dna and induction of mutation in the gadd45β gene was investigated in this study. the present investigation revealed that exposure of zebra fish to pesticides and metals induced dna...

Pathogenic Escherichia coli strains cause a wide range of extra intestinal infections including urinary tract infection in humans and colibacillosis in poultry. They are classified into uropathogenic E. coli (UPEC) and avian pathogenic E. coli (APEC) with genetic similarities and variations. Their pathogenicity is related to the virulence-encoding genes like sfa</...