The mechanisms of modulating milk production traits remain largely unknown. Based on our previous RNA-seq, DDIT3 was presumed as a novel, promising candidate gene for regulating protein and fat in dairy cattle. To further detect the genetic effect its potential molecular cattle, here, we performed genotype-phenotype association study. Two SNPs, g.-1194 C>T g.-128 C>T, were significantly a...

Abstract Innate lymphoid cells (ILCs) are largely tissue-resident and do not express clonotypic receptors. In humans, ILCs primarily described within the mucosal tissues. However, their development functions in human draining lymph nodes (LN) bone marrow (BM) unknown. Here, we performed single-cell RNA sequencing to unravel tissue-specific transcriptional profiles of 47,287 abdominal thoracic L...

The formation of lymph nodes is a complex process crucially controlled through triggering of LTβR on mesenchymal cells by LTα1β2 expressing lymphoid tissue inducer (LTi) cells. This leads to the induction of chemokines to attract more hematopoietic cells and adhesion molecules to retain them. Here we show that the extravasation of the first hematopoietic cells at future lymph node locations occ...

Cutaneous T-cell lymphoma (CTCL), a heterogeneous group of extranodal non-Hodgkin’s lymphoma. In this study, we sequenced skin T cells on single cell level from patients with mycosis fungoides (MF), the most common type CTCL, and segregated clonal malignant non-clonal bystander by natural clonality identifier – receptor (TCR). Based our attention-ANN, an artificial intelligent method, identifie...

Background: The immune tumor microenvironment (TME) is key to controlling disease progression in skin cancer. We aim compare the TME these cancers better inform prognosis and therapeutic decision making. Methods: CD8+ infiltrating lymphocytes (TILs) obtained from fresh immunocompetent SCC (n=5) BCC (n=12) tumors solid-organ transplant recipient (“TSCC”, n=6) were subject single-cell RNA profili...

Radiofrequency radiations constitute a new form of environmental pollution. Among them, millimeter waves (MMW) will be widely used in the near future for high speed communication systems. This study aimed therefore to evaluate the biocompatibility of MMW at 60 GHz. For this purpose, we used a whole gene expression approach to assess the effect of acute 60 GHz exposure on primary cultures of hum...

Activation-induced cytidine deaminase (AID) is essential for somatic hypermutation and class switch recombination in mature B-cells, while AID was also shown to play a role in developing pre-BCR/BCR-positive B-cells of the bone marrow. To further elucidate a potential function of Aid in the bone marrow prior to V(D)J-recombination, we utilized an in vivo model which exerts a B-cell developmenta...

Growth factor independent 1 (Gfi1) is a transcriptional repressor originally identified as a gene activated in T-cell leukemias induced by Moloney-murine-leukemia virus infection. Notch1 is a transmembrane receptor that is frequently mutated in human T-cell acute lymphoblastic leukemia (T-ALL). Gfi1 is an important factor in the initiation and maintenance of lymphoid leukemias and its deficienc...