Many differentially expressed genes associated with feather colour in quail embryos can be screened using RNA-seq technology. The aim of this study was to verify the accuracy RT-qPCR. transcriptome embryonic skin tissues quails were sequenced by Illumina HiSeq TM2000 sequencing platform. As a result, 2512 found. Among them, 950 up-regulated white-feather embryos, and 1562 down-regulated....

BACKGROUND The oriental river prawn, Macrobrachium nipponense, is an important aquaculture species in China, even in whole of Asia. The androgenic gland produces hormones that play crucial roles in sexual differentiation to maleness. This study is the first de novo M. nipponense transcriptome analysis using cDNA prepared from mRNA isolated from the androgenic gland. Illumina/Solexa was used for...

This is the first report of a transcriptome assembly newly discovered hydrothermal vent mussel, Gigantidas vrijenhoeki (Bivalvia: Mytilidae), on Central Indian Ridge. was identified from material collected at Onnuri Vent Field (OVF) Ridge in 2018, and reported as new species, distinct another dominant Bathymodiolus marisindicus, 2020. We sequenced G. using Illumina HiSeq X System. De novo analy...

Next-generation sequencing plays a central role in the characterization and quantification of transcriptomes. Although numerous metrics are purported to quantify the quality of RNA, there have been no large-scale empirical evaluations of the major determinants of sequencing success. We used a combination of existing and newly developed methods to isolate total RNA from 1115 samples from 695 pla...

Abstract Soil bacterial play special roles in the maintenance and stabilization of wetland ecosystem. To investigate community structure diversity rhizosphere soils four dominant plants riparian wetlands mountain reservoir Zhejiang province, V4+V5 areas 16S rDNA gene bacteria were sequenced analyzed by Illumina HiSeq 2500 high-throughput sequencing technology. The results indicated that 27 phyl...

To date, very large scale sequencing of many clinically important RNA viruses has been complicated by their high population molecular variation, which creates challenges for polymerase chain reaction and sequencing primer design. Many RNA viruses are also difficult or currently not possible to culture, severely limiting the amount and purity of available starting material. Here, we describe a s...

BACKGROUND Platelets are small anucleate cells circulating in the blood vessels where they play a key role in hemostasis and thrombosis. Here, we compared platelet RNA-Seq results obtained from polyA+ mRNA and rRNA-depleted total RNA. MATERIALS AND METHODS We used purified, CD45 depleted, human blood platelets collected by apheresis from three male and one female healthy blood donors. The Ill...