The report of Raatz, Böcking and Hauptmann [1] represents an excellent example of an in depth retrospective study on the influence of different clinico-pathological parameters including static DNA cytome-try on tumour prognosis illustrating the power but also some limitations of a comprehensive survival analysis in surgical pathology. The authors studied in total 44 neuroendocrine (car-cinoid) ...

In several tissues, nuclear differences have been described in normal-appearing cells from patients with invasive carcinomas compared to cases without invasive carcinoma, a phenomenon known as malignancy-associated changes (MACs). The aim of this study was to determine the presence of malignancy-associated changes in breast tissue. Image cytometry was performed on Feulgen stained tissue section...

The purpose of this study was to improve the detection of FISH signals, in order that spot counting by a fully automated image cytometer be comparable to that obtained visually under the microscope. Two systems of spot scoring, visual and automated counting, were investigated in parallel on stimulated human lymphocytes with FISH using a biotinylated centromeric probe for chromosome 3. Signal ch...

Flow cytometric assays for apoptosis are now in widespread use. The multiparametric nature of flow cytometry allows multiple assays for several apoptotic characteristics to be combined in a single sample, providing a powerful tool for elucidating the complex progression of apoptotic death in a variety of cell types. This chapter describes one such assay, allowing simultaneous analysis of caspas...

Introduction and Overview The majority of laser types in current use produce output beams with circular or elliptical cross-sections, with either Gaussian or near-Gaussian intensity profiles. This Gaussian intensity distribution is acceptable, and often beneficial for many applications in which the laser beam is being focused to a small spot. However, there are also many different uses for whic...

Cytotoxicity assessment of silver nanoparticles (AgNPs) was performed using MTT-based microfluidic image cytometry (μFIC). The LC50 value of HeLa cells exposed to AgNPs in the microfluidic device was estimated as 46.7 mg/L, which is similar to that estimated by MTT-based IC for cells cultured in a 96 well plate (49.9 mg/ L). These results confirm that the μFIC approach can produce cytotoxicity ...

BACKGROUND The sensitivity for identification of malignant cells in conventional fine-needle aspiration biopsy (FNAB) investigation is about 80%. This percentage is dependent on the number of examined cells, type of breast cancer, and experience of the examiner. The aim of our study was to estimate the supporting value of image DNA cytometry of FNAB of the breast, and do so by using different s...

Retinopathic injuries are a common symptom of many diseases. However, if detected early, much of the damage caused by these injuries can be prevented, or in some cases reversed. In this study, images of retinas were classified as normal or injured using the vascular cell count, vasculature coverage, and vessel caliber. To model retinal vasculopathies, retinal vasculature from mice with the BCL-...