Although the efficacy of computer assisted sperm analysis (CASA) has been demonstrated for use with a variety of species in assessing male reproductive quality as well as the impact of various treatments on sperm motility, many investigations continue to rely on manual analytical methods. CASA has been consistently shown to outperform such methods in terms of reproducibility as well as in the v...

The current version allows for five different PSF models: The Gaussian model simulates a blurring effect by setting three different variance values. These values characterize the width of the PSF at various axial positions along the z-stack. The defocus model is simulated in the Fourier domain by a modulated Gaussian where the sinc modulation depends on the axial defocusing distance. Another de...