Alcohol dehydrogenase from Bacillus (Geobacillus) stearothermophilus (BsADH) is a NADH-dependent enzyme catalyzing the oxidation of alcohols, however its thermal and operational stabilities are too low for long-term use under non-physiological conditions. Enzyme immobilizations emerges as an attractive tool to enhance stability this enzyme. In work, we have screened battery porous carriers immo...

The citric acid excretion of Ca-alginate-immobilized cells of Aspergillus niger in batch culture decreased with a half-time of approximately 19 days. Reactivation of the biocatalysts by regeneration in growth medium was possible, but it was followed by a submerged sporulation of the fungus, and medium was highly contaminated with free cells. Citric acid production could better be prolonged by s...

Sol-gel immobilization of enzymes, which can improve considerably their properties, is now one of the most used techniques. By deposition of the entrapped lipase on a solid support, a new and improved biocatalyst was obtained, which can be used with excellent results in acylation reactions. In this paper, lipase B from Candida antarctica was double immobilized on different adsorbents. These bio...

Although the current literature describes significant advances in biocatalytic ester syntheses, few industrial plants worldwide are currently producing esters using biocatalysts. Green and sustainable can be obtained via a route, including some operational advantages over conventional syntheses. An analysis of revealed that most articles neglect or describe economic issues generically, without ...

Recent years have witnessed a growing interest in the use of biocatalysts flow reactors. This merging combines high selectivity and mild operation conditions typical biocatalysis with enhanced mass transfer resource efficiency associated to chemistry. Additionally, it provides sound environment emulate Nature by mimicking metabolic pathways living cells produce goods through systematic organiza...

Immobilization process is to optimize the operational performance of an enzyme for industrial applications. So far different matrices have been described in the literature to improve the performance of the immobilized enzymes. With the advent of nanotechnology, the nanomaterials because of their unique physico-chemical properties constitute novel and interesting matrices for enzyme immobilizati...

The polyphosphazene {NP[O(2)C(12)H(7.5)(NH(2))(0.5)]}(n), prepared by reacting {NP[O(2)C(12)H(7.5)(NO(2))(0.5)]} with the Lalancette's reagent, was used for attaching enzymes such as alcohol dehydrogenase (ADH-A) and lipase (CAL-B). The resulting new biocatalysts exhibited great potential as tunable supports for enzymatic reactions in both aqueous and organic media. The material with immobilize...

Because of their high volumetric catalytic activity, in addition to chemical and thermal resistances, enzymes the form protein crystals are an excellent choice for application as immobilized biocatalysts. However, mechanical stability is a requirement processability immobilisates, retaining enzymatic this closely related crystal structure. In study, influence engineering on cross-linked enzyme ...