Introduction: The measurement of glycated hemoglobin (HbA1c) is an established procedure in evaluating long-term glycemic control diabetic patients. There are many factors like temperature, pH, lifespan protein, substrate concentrations and levels that influence HbA1c estimation by any methods. aim the study to estimate High Performance Liquid Chromatography (HPLC) immunochromatography analyzer...

background: canine parvovirus (cpv) infection is one ofthe most common causes of infectious gastroenteritis in dogs andis a highly contagious, often fatal disease. the original virus(cpv type 2) has had some mutations since its emergence andnew variants (cpv-2a, 2b and 2c) have been reported from manycountries all around the world. early diagnosis and treatment canprofoundly affect the disease ...

canine parainfluenza virus (cpiv) is one of the most common organisms isolated from dogs with signs of infectious tracheobronchitis (itb). distribution is apparently worldwide. although cpiv may cause mild clinical infections, clinical diseases is expected to be more severe in dogs co-infected with bordetella bronchiseptica than with any these agents alone. the present study was conducted to de...

BACKGROUND Escherichia coli O157:H7 (E. coli O157:H7) is an important pathogenic bacterium that threatens human health. A rapid, simple, highly sensitive, and specific method for the detection of E. coli O157:H7 is necessary. METHODS In the present study, immunomagnetic nanoparticles (IMPs) were prepared with nanopure iron as the core, coated with E. coli O157:H7 polyclonal antibodies. These ...

Immunochromatography (IC) is widely used to detect target molecules in biological fluids. Since this method can be performed without a special technique or device, IC is a convenient way to assess the existence of antibodies or pathogens such as viruses and bacteria, simply and quickly. In this study, we established an IC method to detect serum antibodies against oncogenic human papillomavirus ...

We recently demonstrated how various enveloped viruses can be efficiently concentrated using magnetic beads coated with an anionic polymer, poly(methyl vinyl ether-maleic anhydrate). However, the exact mechanism of interaction between the virus particles and anionic beads remains unclear. To further investigate whether these magnetic anionic beads specifically bind to the viral envelope, we exa...

Improvement of the methods included in the World Organisation for Animal Health (OIE) Manual of Diagnostic Tests for Aquatic Animals and addition of novel techniques are dependent on the continual development and evaluation of both new and existing methods. Although conventional isolation and characterisation techniques for the diagnosis of many diseases still remain the methods of choice there...

One of the measures to control visceral leishmaniosis (VL) in Brazil is the identification and culling of the canine reservoir. There is much controversy concerning this strategy, including the proper identification of positive dogs and the fact that the host-parasite relationship changes over time make it more challenging. A dynamic cohort of 62 dogs was followed every three months using serol...

background: acute gastroenteritis is responsible observed in all age groups, especially infants and children. the etiology and clinical course of acute gastroenteritis may vary with age and etiological agents. in developing countries, the morbidity and mortality associated with infectious diarrhea is higher in children younger than five-years-of-age. objectives: the aim of this study was to det...