A new commercially available rapid screening immunochromatographic test (ICT) for Detection of anti-rubella IgG & IgM antibodies was compared with ELISA. Materials and methods: 161 serum samples were tested by immunochromatography card test (Rubella IgG/IgM, SD –Bioline) and were compared with a standard IgG & IgM ELISA (Ani Biotech Oy, Orgenium Laboratories, Vantaa, Finland). Results: ELISA an...

BACKGROUND Leptospirosis is a re-emerging infectious disease that is under-recognized due to low-sensitivity and cumbersome serological tests. MAT is the gold standard test and it is the only serogroup specific test used till date. Rapid reliable alternative serogroup specific tests are needed for surveillance studies to identify locally circulating serogroups in the study area. METHODS/PRINC...

A perforated-patch whole-cell recording method was used to determine whether nitric oxide signaling participates in acetylcholine (ACh)-induced regulation of basal L-type Ca2+ current (ICa,L) in cat atrial myocytes. Exposure to 1 microM ACh for 2 min inhibited basal ICa,L (-21 +/- 3%), and withdrawal of ACh elicited rebound stimulation of ICa,L above control (80 +/- 13%) (n = 23). Stimulation o...

Staphylococcus aureus is a major hospital and community pathogen having the aptitude to cause a wide variety of infections in men. The ability of microorganisms to produce biofilm facilitates them to withstand the host immune response and is recognized as one factor contributing to chronic or persistent infections. It was demonstrated that the ica-encoded genes lead to the biosynthesis of polys...

Acute diarrheal illness is a serious public health issue in developing and developed economies, with high morbidity mortality rates. Children below five years are the most vulnerable, one every six dying from diarrhea. Diarrhea may be caused by various viruses, protozoa, bacteria. Rotavirus, particular, often associated diarrhea children. Early detection of rotavirus infection reduces need for ...

Bone mesenchymal stem cells (BMSCs) are able to differentiate into multi types of lineages, so they have been widely applied in the stem cell transplantation. The BMSCs are usually needed to be expanded before transplantation due to their limited content in bone marrow. It has recently been reported that Icariin (ICA), a major constituent of flavonoids from the Chinese medical herb Epimedium br...

A dipstick dye immunoassay (DDIA) was developed to detect immunoglobulin G (IgG) or IgM antibodies of toxoplasmosis infection in humans. The assays employ a blue colloidal dye particles (D-1) conjugated to sheep anti-human IgG and rabbit anti-human IgM as the visualizing agents and a soluble antigen of tachyzoites of Toxoplasma gondii strain RH (TSA) as the detective antigen. The mixture of dye...

introduction: the accuracy of analyzing functional mri (fmri) data is usually decreases in the presence of noise and artifact sources. a common solution in for analyzing fmri data having high noise is to use suitable preprocessing methods with the aim of data denoising. some effects of preprocessing methods on the parametric methods such as general linear model (glm) have previously been evalua...

Two male dogs at the age of 3 and 5 months of Doberman pinscher and German shepherd breeds were referred to the Veterinary Hospital of Ahvaz University with clinical signs of depression, vomiting, haemorrhagic diarrhoea, profound dehydration, fever and anorexia. The affected dogs had not the history of vaccination. Concurrent infection of canine parvovirus and coronavirus was detected in dogs b...