The addition of canavanine to cultures of superinfected Raji cells in the absence of arginine prevented the appearance of early antigens as defined by immunofluorescent staining. Addition of various amounts of arginine permitted the identification of at least three groups of proteins, each responding differently to the various concentrations of arginine-canavanine.

BACKGROUND The uterus is a dynamic tissue responding to hormonal changes during reproductive cycles. As such, uterine stem cells have been studied in recent years. Transcription factors oct4 and sox2 are critical for effective maintenance of pluripotent cell identity. OBJECTIVE The present research evaluated the mRNA expression of oct4 and sox2 in the uterine tissues of ovariectomized mice tr...

The unusual cell type present on the posterior corneal surface of posterior polymorphous dystrophy patients has been characterized. In addition to microvilli and desmosomes, these cells contain abundant 10 nm filaments which by immunofluorescent staining were shown to consist of keratin proteins, a marker for epithelial cells.

A splenectomized aotus monkey infected with human quartan malaria (Plasmodium malariae) developed oedema and proteinuria. Histological examination revealed a generalized diffuse glomerulonephritis and immunofluorescent staining showed granular deposits of IgM in the glomeruli. The pathological picture resembled that shown by human patients with the quartan malaria nephrotic syndrome.

OBJECTIVES The present study aimed at developing tissue-engineered mitral valves based on cell-free ovine mitral allografts. METHODS The ovine mitral valves (OMVs) (n = 46) were harvested in the local slaughter house. They were decellularized using detergent solutions and DNase. The effectiveness of decellularization was assessed by histological (haematoxylin-eosin, Movat's pentachrome) and i...

Antibodies against chicken and Torpedo agrin were used for immunofluorescent staining in order to assess the spatial distribution and temporal appearance of agrin-like molecules at newly formed synaptic contacts in cultures of embryonic Xenopus nerve and muscle cells. The antibodies stained Xenopus neuromuscular junctions and removed ACh receptor (AChR)-aggregating activity from extracts of Xen...

Factor-VIII-related antigen has previously been shown to be synthesized by vascular endothelial cells. Using both an immunofluorescent staining technique and electron microscopy, we have demonstrated the presence of factor-VIII-related antigen in human vascular subendothelium. This finding may have implications in the mechanism of platelet adhesion to deendothelialized blood vessel surfaces.

Objectives To observe changes in actin and dendritic spines the hippocampus after propofol anesthesia, to evaluate role of these subsequent learning impairment both young (3-month-old) aged (20-month-old) male rats. Methods The shuttle box test was used from 1, 3, 7, or 14 days anesthesia. Both F-actin content hippocampal CA1 region were observed using immunofluorescent staining western blot as...

Background: The purpose of this study was to create biomaterial scaffolds like platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) containing stromal cell-derived factor-1 (SDF1) as a chemokine to induce hyaline cartilage regeneration of rabbit knee in a full thickness defect.Methods: We created a full thickness defect in the trochlear groove of thirty-six bilateral knees of eighteen matu...

In this study, it was aimed to examine Canine Herpesvirus-1 (CHV-1) with different diagnostic methods and investigate the pathological changes in positive samples. For purpose, 100 dog uteruses of ages breeds, were removed by ovariohysterectomy their blood samples used as study material. Enzyme-linked immunosorbent assay (ELISA) Indirect Fluorescent Antibody Test (IFAT) for serological diagnosi...