Background Breast cancer is a common malignant tumour prevalent in Ireland and rest of the world. Although breast cancer screening and treatment has dramatically improved patient prognosis, it is still the leading cause of cancerrelated death for women. One of the major bases for breast cancer treatment is patient’s receptor status: ER, PR, HER2 positive or negative. Immunohistochemistry (IHC) ...

Right detection of anaplastic lymphoma kinase (ALK) gene rearrangement is pivotal to selection of patients with lung adenocarcinoma for ALK-targeted therapy. We explored the potential of combination of immunohistochemistry (IHC) screening and fluorescence in situ hybridization (FISH) as an affordable practice. We analyzed 410 unselected lung adenocarcinomas by ALK IHC (D5F3 clone) and FISH. Som...

BACKGROUND Determination of Her-2/neu overexpression in breast cancer has previously been shown to be of prognostic significance. In this study, Her-2/neu expression in breast cancer was characterised by real-time PCR (RLT-PCR) based LightCycler-HER-2/neu DNA Quantification with immunohistochemistry (IHC) and fluorescence in situ hybridisation (FISH). MATERIAL AND METHODS Fifteen specimens of...

FAT is a gold standard and validated diagnostic test that confirm the presence of rabies virus in brain tissue. However, sophisticated laboratory infra-structure and highly trained personnel are required for conducting rabies diagnosis by immunofluorescence. On the other hand, immunohistochemistry (IHC), with its immunological approach of antigen detection is expected to be close to FAT in sens...

PURPOSE V600E is the most common activating BRAF mutation in colorectal carcinomas (CRCs). It is a crucial biomarker for patient selection and response to targeted therapy with BRAF V600E inhibitors. Previous studies using immunohistochemistry (IHC) have shown different results. In this study, we evaluated the IHC expression of the mutated BRAF protein in archival material from CRC specimens an...

BACKGROUND The expression of pregnancy-associated plasma protein A (PAPP-A) was identified by immunohistochemistry (IHC) in culprit atherothrombotic plaque specimens harvested from patients admitted with ST-segment elevation myocardial infarction (STEMI). METHODS The atherothrombotic samples were collected from a consecutive cohort consisting of 20 individuals admitted with STEMI to Stavanger...

The use of immunohistochemistry (IHC) in clinical cohorts is of paramount importance in determining the utility of a biomarker in clinical practice. A major bottleneck in translating a biomarker from bench-to-bedside is the lack of well characterized, specific antibodies suitable for IHC. Despite the widespread use of IHC as a biomarker validation tool, no universally accepted standardization g...

p53 mutations have been linked with shortened survival rates in non-small cell lung cancer (NSCLC). Hypermethylation of RASSF1 and DAPK1 genes, which are downstream targets of p53, has also been linked to a poor prognosis in lung cancer patients. We investigated whether p53 mutations, assessed as p53 stabilization by immunohistochemistry (IHC), were independent of DAPK1 and RASSF1 promoter hype...

Merkel cell polyomavirus (MCPyV) is an oncogenic virus etiologically related to carcinoma and has been found in association with non-melanoma skin cancer (NMSC), controversial results. Little known about the role or influence of MCPyV development these tumors its prevalence various populations. To assess MCPyV`s Large T Antigen DNA cases NMSC, nested-PCR immunohistochemistry (IHC) were performe...

BACKGROUND To evaluate the diagnostic value of a novel fully automated immunohistochemistry (IHC) assay for detection of anaplastic lymphoma kinase (ALK) fusion in a large number of ALK-positive lung adenocarcinoma (ADC) patients. PATIENTS AND METHODS We tested 196 lung ADCs for ALK rearrangement by two IHC assays (Ventana pre-diluted ALK D5F3 antibody with the Optiview DAB IHC detection kit ...