Many different radiolabeled antibodies have been used for radioimmunotherapy and radioimmunoscintigraphy of human diseases in animal experiments. In order to study the in vivo tissue distribution of antibody, we labeled human nonspecific polyclonal IgG with Na125I using chloramine-T method. An animal model was developed by injecting turpentine in the posterior left thigh of Balb/c mice. Tissue ...

Many different radiolabeled antibodies have been used for radioimmunotherapy and radioimmunoscintigraphy of human diseases in animal experiments. In order to study the in vivo tissue distribution of antibody, we labeled human nonspecific polyclonal IgG with Na125I using chloramine-T method. An animal model was developed by injecting turpentine in the posterior left thigh of Balb/c mice. Tissue ...

Monoclonal antibodies (mAbs) play a significant part in the diagnostic and therapeutic arsenal. They are used in many fields of medicine, ranging from in vitro diagnosis to the treatment of multiple pathologies in such diverse areas as infectious diseases, immunology and oncology. The first mention of the use of radiolabeled antibodies dates from the 1950s. However, from the first tests, severa...

CASE A 65-year-old man presented for consultation because his prostate-specific antigen (PSA) level was elevated. The patient’s medical history is significant for a radical retropubic prostatectomy 11 years ago. Before the surgery, the patient’s serum PSA level was 3.0 ng/mL. Adenocarcinoma of the prostate was diagnosed by biopsy. The pelvic lymph nodes were negative at the time of the surgery;...

Willm Uwe Kampen, MD, MS; Clinic of Nuclear Medicine, Kiel University Hospital, Germany Imaging of inflammation and infection is a broad field of nuclear medicine diagnostics. Many radiotracers are approved for the multitude of possible pathogenetic reasons and all of them show several advantages and disadvantages compared to others in special clinical questions. Gallium-67, for example, is exc...

Using viable cells of a human head and neck squamous cell carcinoma (HNSCC) cell line as immunogen, we developed monoclonal antibody (MAb) U36. Immunohistochemical examination revealed distinct surface labeling of MAb U36 with normal human squamous epithelium and squa mous cell carcinomas of distinct sites of origin; head and neck, lung, esophagus, cervix, and epidermis. MAb U36 shows high affi...

A Phase I dose-escalation study using #{176}Y-CYT-356 monoclonal antibody was performed in 12 patients with hormone-refractory prostate carcinoma. Biodistribution studies using “In-CYT-356 were performed 1 week before #{176}Y-CYT-356 administration. Of the 12 patients, 58% had at least one site of disease imaged after administration of’ “InCYT-356. The dose of #{176}Y ranged from 1.83-12 mCi/m2...

Monoclonal antibodies (MAbs) against high-molecularweight melanoma-associated antigen (HMW-MAA) have been used in vivo to target melanoma. More recently, single chain Fv (scFv) antibody fragments against HMW-MAA have been described that may improve melanoma targeting. However, there have been few in vivo studies with antimelanoma scFvs because these have proved difficult to label with isotopes ...