By using the methodology of both wet and dry biology (i.e., RT-PCR and cycle sequencing, and biocomputational technology, respectively) and the data obtained through the Genome Projects, we have cloned Xenopus laevis SOD2 (MnSOD) cDNA and determined its nucleotide sequence. These data and the deduced protein primary structure were compared with all the other SOD2 nucleotide and amino acid seque...

It is well known that it impossible to clone an arbitrary quantum state. However, this inability does not lead directly no-cloning of coherence. Here, we show the coherence state which a stronger statement than 'no-cloning state'. In particular, with ancillary system as machine state, states whose greater on transformations are defined. Also, characterize class for cloning will be possible give...

with regard to human cloning or artificial human reproduction – and contrary to the opinions of sunni scholars - shiite thinkers have not held a unified position. after having surveyed a number of shiite fatwas and analyses on the subject, this essay will classify them into four groups. the first group states that we are granted absolute permission to engage in human cloning; while the second g...

Introduction: The design and structural characterization of mini-proteins with a compact, folded structure provide insight into the complex architecture of proteins today and has long been a challenging issue in structural- functional studies. Alpha neurotoxins from snake venom have a distinct folded structure comprised of a disulphide core and three loops or “fingers” each of these loops are c...

β -Propeller phytases (BPPhy) are widely distributed in nature and play a major role in phytate-phosphorus cycling. In the present study, a BPPhy gene from Bacillus licheniformis strain was expressed in E. coli with a phytase activity of 1.15 U/mL and specific activity of 0.92 U/mg proteins. The expressed enzyme represented a full length ORF "PhyPB13" of 381 amino acid residues and differs by 3...

We had previously reported presence of histone deacetylase 6 (HDAC6) in sperm and demonstrated its tubulin deacetylase activity and role in sperm motility in rat. In the present study we report its abundant expression in testis, epididymis, accessory sex organs, brain, and adrenal. In the testis, HDAC6 transcript and protein were observed throughout development. We therefore cloned the gene fro...