Degradation of dry matter (DM) and nitrogen (N) in fresh or frozen-thawed alfalfa and ryegrass was studied by using in situ technique. The forages were labeled with 15N during growth in a glasshouse, harvested at similar growth phase, and fresh (F) or frozen-thawed (FT) samples were incubated in the rumen of 3 sheep. There was no difference (P>0.05) between forage type (S) for the immediately s...

Three techniques for estimating rumen protein degradability were used together with in vivo apparent nitrogen digestibility estimates from sheep fed dried chopped Pangola grass or Siratro. The techniques used were 1) nitrogen disappearance from nylon bags suspended in the rumen, 2) S labelling of rumen microbial protein with plant nitrogen degradability calculated as the difference between micr...

despite the great potential of graphene as a nanofiller, achieving homogeneous dispersion remains the key challenge for effectively reinforcing polyolefin (such as polyethylene (pe) and polypropylene (pp)) nanocomposites. therefore, in this research, we report a facile combined in situ polymerization and masterbatch method for fabricating pp/reduced graphene oxide (rgo) nanocomposites. in the p...

Introduction The in situ method is used widely to determine ruminal degradability of feed CP and DM. It is an attractive alternative to in vivo methods because it is simple, inexpensive, and rapid. However, several factors influence in situ results, the most important of which is microbial contamination of feed residues after incubation in the rumen. Degradation of forage CP and DM is systemati...

The objective of this study was to determine the effect of the heat treatment of full-fat soyabean (FFSB) and solvent extracted soyabean meal (SBM) on the in situ dry matter (DM) and protein degradability, and in vitro gas production kinetics of the protein sources. Ruminal disappearance of DM and crude protein (CP), and in vitro gas production were determined after 0, 4, 8, 16, 24, 48 and 72 h...

The objectives were to compare the effect of exogenous fibrolytic enzyme (Biocellulase A20) or anhydrous ammonia (4% DM) treatment on the nutritive value, voluntary intake, and digestion kinetics of bermudagrass (Cynodon dactylon cultivar Coastal) hay harvested after 2 maturities (5- and 13-wk regrowths). Six individually housed, ruminally cannulated Brangus steers (BW 325 ± 10 kg) were used in...

Rumen degradation kinetics and effective degradability of individual amino acids, total analysed amino acids (TAA) and crude protein (CP) of soybean meal were measured on four rumen-cannulated wethers using the nylon bag technique. Microbial contamination of the incubated residues was corrected using a continuous 15N intraruminal infusion and isolated solid associated bacteria as a reference sa...