To develop an alternative method for production of sarsasapogenin by plant tissue culture technique and its quantification in Asparagus racemosus and its in vitro cultures using HPTLC. Murashige and Skoog’s (MS) basal medium supplemented with various growth regulators was used for development of shoot and root calli while, hormone free MS medium was used for development of Agrobacterium tumefac...

BACKGROUND Aloe vera L. is an important pharmaceutical plant from which several medicinal and cosmetic compounds are extracted. Aloe is naturally propagated through offset, which is a slow and expensive labor cost method with low economical income. OBJECTIVES In this study, the effect of different media on shoot proliferation of the shoot tip of Aloe vera L. was investigated. MATERIALS AND ...

A reliable in vitro propagation protocol has been established from nodal segments of a highly valuable medicinal plant Solidago virgaurea L. The explants were cultured on Murashige and Skoog’s medium augmented with different concentrations and combinations of plant growth regulators for shoot bud initiation and multiplications. Highest frequency of shoot proliferation (91.8 ± 0.64) and maximum ...

To develop an alternative method for production of sarsasapogenin by plant tissue culture technique and its quantification in Asparagus racemosus and its in vitro cultures using HPTLC. Murashige and Skoog’s (MS) basal medium supplemented with various growth regulators was used for development of shoot and root calli while, hormone free MS medium was used for development of Agrobacterium tumefac...

A rapid and efficient protocol was developed for inducing indirect organogenesis using leaf explants of Vitex negundo L. Explants were cultured on MS with different concentrations of 2,4-D and IAA in combination with BAP for callus induction. The frequency of callus induction increased with increasing concentration of IAA (0.3 mg/l) and BAP (0.3 mg/l) at optimal level. The shoot buds appeared e...

this research was aimed to investgate the effect of light quality on micropropagation of pear rootstock oh×f333. first, single nodes explants were cultured in modified ql medium containing ba at four levels (0, 0.5, 1, and 2 mg/l-1) and iaa at three levels (0, 0.1, and 1 mg/l-1) and were evaluated for shoot proliferation. then, plantlets subcultured in modified ql medium containing 1 mg/l-1 ba ...

Polyploid plantlets, including triploid, tetraploid, and mixoploid, were induced from the European pear (Pyrus communis L.) cultivar ‘Fertility’ by in vitro colchicine treatment of leaf explants. The leaf explants were incubated in 0.4% (w/v) colchicine for 24, 48, or 72 h, then transferred to adventitious shoot-induction medium. Regenerated shoots were pre-selected according to their morpholog...

In vitro culture has the greatest commercial value in extremely quick creation of clonal plants compared to conventional techniques propagation. It also proven be tremendous practical utility as assistance development and maintenance disease-free planting material, germplasm conservation, supplements regular methods plant progress, such genetic engineering. The discovery implementation current ...

In vitro propagation method was developed for obtaining large number of plantlets of Valeriana wallichii DC. (Valerianaceae), an indigenous high value medicinal and aromatic plant species of Indian Himalayan region, using nodes of in vitro grown seedlings. High frequency shoot proliferation was induced in explants cultured on Murashige and Skoog (MS) medium supplemented with different concentra...