In vivo optical imaging can reveal the dynamics of large-scale cortical activity, but methods for chronic recording are limited. Here we present a technique for long-term investigation of cortical map dynamics using wide-field ratiometric fluorescence imaging of the genetically encoded calcium indicator (GECI) Yellow Cameleon 3.60. We find that wide-field GECI signals report sensory-evoked acti...

To understand ecological systems and environmental impact, techniques can be used which combine thermodynamics with environment and ecology. Most assessments consider thermodynamics via energy, but it many feel that ecological and environmental factors are better understood through exergy. One rationale is that exergy, not energy, is often a measure of the potential for ecological and environme...

This study discusses the fusion of chlorophyll-a (Chl.a) estimates around Tachibana Bay (Nagasaki Prefecture, Japan) obtained from MODIS and GOCI satellite data. First, the equation of GOCI LCI was theoretically calculated on the basis of the linear combination index (LCI) method proposed by Frouin et al. (2006). Next, assuming a linear relationship between them, the MODIS LCI and GOCI LCI meth...

Astrocytes generate calcium signals throughout their fine processes, which are assumed to locally regulate neighbouring neurotransmission and blood flow. The intercellular morphological relationships mature during juvenile periods when astrocytes elongate highly ramified processes. In this study, we examined developmental changes in calcium activity patterns of single hippocampal astrocytes usi...

The e-business ecosystem may be deviate from the equilibrium state in its development process. In this article the authors defined the e-business ecosystem health by combining ecological principles, and developed ecological indicators of e-business ecosystem health. The method of ecosystem health assessment (include biodiversity index method and index of biological integrity method) was introdu...

Calcium imaging using fluorescent reporters is the most widely used optical approach to investigate activity in intact neuronal circuits with single-cell resolution. Calcium signals, however, are often difficult to interpret, especially if the desired output quantity is membrane voltage or instantaneous firing rates. Combining dendritic intracellular electrophysiology and multi-photon calcium i...

Combining intracellular electrophysiology and multi-photon calcium imaging in vivo, we studied the relationship between calcium signals (sampled at 500-750 Hz) and spike output in principal neurons in the locust antennal lobe. Our goal was to determine whether the firing rate of individual neurons can be estimated in vivo with calcium imaging and, if so, to measure directly the accuracy and res...

In this study we present an application which can be accessed via www.excellence-networks.net and which represents networks of scientific institutions worldwide. The application is based on papers (articles, reviews and conference papers) published between 2007 and 2011. It uses (network) data, on which the SCImago Institutions Ranking is based (Scopus data from Elsevier). Using this data, inst...

This document specifies Dynamic Host Configuration Protocol options (both DHCPv4 and DHCPv6) for the coordinate-based geographic location of the client. The Location Configuration Information (LCI) includes Latitude, Longitude, and Altitude, with resolution or uncertainty indicators for each. Separate parameters indicate the reference datum for each of these values. This document obsoletes RFC ...