background: it was shown that olive leave extract has antifungal, antibacterial and antiviral activities. the effects of ole on herpes simplex virus-1 (hsv-1) have not been systematically investigated yet. the aim of this study was to examine the in vitro effect of olive leaf hydroalcoholic extract (olhe) on hsv-1.   methods: virucidal effect and viral replication in vero cell line were studied...

background and purpose: microsporidiosis is one of the emerging and opportunistic infections, which causing various clinical symptoms in humans. the prevalence of this infection varies, depending on the infected organ, diagnostic methods, and geographical conditions. in the present study, we aimed to investigate microsporidial keratitis in patients referring to farabi eye hospital tehran, iran ...

due to the number of new substances coming into use every year and the increasing amounts of chemicals, which are introduced into the environment, there is a high demand for a rapid, reliable and cost-effective method for detection of developmental toxicity. to meet this challenge various in vitro techniques have been established additional to in vivo animal testing. this review introduces the ...

objective: mycoplasma salivarium (m. salivarium) isone of the most common contaminants present in cell culture laboratories that cause undesirable effects on cell cultures. thus, the identification and rapid diagnosis in controlling and prevention of this contaminant are important. the aim of this study is the detection of mycoplasma salivarium contamination in cell culture using polymerase cha...

background: in vitro model studies are becoming increasingly popular for experimental research designs. they include isolation and expansion of cells of a particular tissue, such as the nervous tissue which contributes to understanding the underlying mechanisms in many pathologies. it enables  the scrutinization of intracellular signaling pathways responsible for cell death. objectives: in the ...

background and objectives: contamination of cell lines and biological products is one of the major problems of cell culture techniques. rapid detection of mycoplasma contamination in cell culture is an important part of quality control standards in related laboratories. the aim of this study was to evaluate the efficacy of pcr in detection of myroplasma as contaminants in cell cultures and othe...

introduction: antibiotic supplements are regularly used in neuronal culture media to control contamination however, they can interfere with the neuronal excitability and affect electrophysiological properties. therefore, in this study, the effect of penicillin/streptomycin supplements on the spontaneous electrophysiological activity of hippocampal pyramidal neurons was examined. methods: electr...

The aim of the present work was to examine the immunopharmacological (anti-microbial and anti-inflammatory) effect of saponin on human peripheral blood mononuclear cells (PBMC) that are isolated as well as purified from infected (virally) and non-infected (normal) human whole blood samples. However, there is little information available concerning the effect of crude saponin on human PBMC in ca...

Dengue virus type 2 (DENV-2) infection induced apoptotic cellular DNA fragmentation in Vero cells within 8 days of infection. The addition of high concentrations of extracellular Zn(2+) but not Ca(2+), Mg(2+) or Mn(2+) to the cell culture medium hastened the detection of apoptosis to within 4 h after infection. No apoptotic cellular DNA fragmentation was detected in the cell culture treated wit...