Infectious bronchitis is a disease of the upper respiratory tract chickens caused by Gammacoronavirus (infectious virus, IBV). Severe economic losses are IBV due to reduction in egg production and/or quality layers addition poor feed conversion and increased condemnations broiler chickens. The extreme variability this virus part its RNA genome, which predisposes it mutations generates genetic v...

Infectious bronchitis virus (IBV) poses a severe threat to the poultry industry and causes heavy economic losses worldwide. Vaccination is the most effective method of preventing infection and controlling the spread of IBV, but currently available inactivated and attenuated virus vaccines have some disadvantages. We developed a chimeric virus-like particle (VLP)-based candidate vaccine for IBV ...

infectious bronchitis (ib) is a very contagious disease caused by a coronavirus (ibv). in chickens, thevirus affects the respiratory, reproductive, and urinary systems. this study was carried out to determine theseroprevalence of anti-ibv antibodies in domestic village chickens. serum samples of 300 domestic villagechickens from esfahan (centeral iran) were collected and examined for the presen...

Preparation and Evaluation of Master seed prepared from Local variant infectious bronchitis virus (IBV) isolated from different poultry farms in Egypt and identified as IS/1494/06 nephropathogenic strain are very important point for vaccine production for controlling of infectious brobchitis(IB) disease in Egypt. Master seed of the virus (MSV) contained 10 EID50 per dose per bird free from bact...

Infectious bronchitis virus (IBV) can multiply effectively in chick embryo kidney (CEK) cells after adapting to the chick embryo. To investigate the dynamic changes in IBV load in the supernatant of primary CEK cells, we developed an SYBR Green I-based real-time polymerase chain reaction assay to quantify nucleic copy numbers of the IBV-Sczy3 strain. The 20, 54, and 87th generations of CEK-adap...

nucleocapsid (n) protein of infectious bronchitis virus (ibv), one of the viral structural proteins, inducesstrong antibody response in natural infection. in this study, a simple, recombinant n protein-based dot-blottest was developed to serologically examine chicken serum samples for the presence of ibv antibody.initially, 72 serum samples were tested for the presence of ibv antibody using a c...

an indirect enzyme–linked immunosorbent assay (elisa) was developed for screening of antibody to avian infectious bronchitis virus (ibv). antigen was prepared from whole-purified ibv massachusetts serotype (br 801 strain). optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed elisa was determined 0.1μg/ml, 1:3000 and 1:100, respe...

Infectious bronchitis virus (IBV) is distributed worldwide and causes significant losses in the poultry industry. In recent decades, lineages GI-19 GI-7 have become most prevalent IBV strains China. However, molecular evolution phylodynamics of lineage remain largely unknown. this study, we identified 19 from clinical samples January 2021 to June 2022 China, including 12 GI-19, 3 GI-7, 1 strain...

Here we introduce a culture system for the isolation, passaging and amplification of avian tracheal epithelial (ATE) cells. The ATE medium, which contains chicken embryo extract and fetal bovine serum, supports the growth of ciliated cells, goblet cells and basal cells from chicken tracheas on fibronectin- or matrigel-coated dishes. Non-epithelial cells make up less than 10% of the total popula...

Colibacillosis results from infection with avian pathogenic Escherichia coli bacteria. Healthy broilers are resistant to inhaled E. coli, but previous infection with vaccine or virulent strains of Infectious Bronchitis Virus (IBV) predisposes birds for severe colibacillosis. The aim of this study was to investigate how IBV affects the course of events upon infection with E. coli. Broilers were ...