a new sensitive, simple and rapid method for determination of methotrexate (mtx) was developed based on quenching effects of mtx on the fluorescence intensity of tb3+-1,10-phenanthroline complex. the fluorescence intensity was measured with excitation wavelength of 300 nm and emission wavelength of 545 nm and the quenched fluorescence intensity is proportional to the concentration of mtx in tris...

In magnetic resonance imaging (MRI), image intensity inhomogeneity hampers the quantitative analysis, e.g. segmentation or registration. Intensity inhomogeneity is an effect perceived as a smooth variation in intensities across the image. This study provides an efficient, fully automated intensity inhomogeneity correction method that makes no a priori assumptions on the image intensity distribu...

It is necessary to calibrate the intensity between stereo images correctly to find their correct correspondence when using a correlation method. The authors propose a method of automatically calibrating the intensity from the image data, using segment-based correspondence results, which are robust against changes in intensity. The effects of this method are explained through experiments.

in this paper, the mixed-mode dynamic fracture analysis of a fgm plate is performed using mlpg method also the effect of changes in value and angle of material gradation on the dynamic stress intensity fctor(sif) of mode i, ii, and effective are investigated. to solve time dependent equations that discretized by mlpg method, the central difference method (cdm) and the newmark method are used. a...

Immunohistochemistry is a widely used technique for detecting the presence, location, and relative abundance of antigens in situ. This introductory level protocol describes the reagents, equipment, and techniques required to complete immunohistochemical staining of rodent brain tissue, using markers for microglia and neuronal elements as an example. Specifically, this paper is a step-by-step pr...

Twenty five to thirty specimens each from ten populations of Mus terricolor of the Terai and the Dooars regions of the Darjeeling foothills of West Bengal were cytogenetically analyzed using C-banding. Results showed intra- and inter- population variation of C-band positive heterochromatin ranging from very large blocks to minute amounts or even complete absence of heterochromatin. Large blocks...

Titrimetric methods are considered to be useful for the study of biological wastewater treatment processes, particularly those processes that have negligible influence on the dissolved inorganic carbon pool. However, the application of titrimetric methods for studying biological processes that produce/consume carbon dioxide is not straightforward as microbial activity affects the total amount o...

BACKGROUND The expression of the insulin-like growth factor (IGF) system has never been studied in gastrointestinal stromal tumors (GISTs). PATIENTS AND METHODS We studied the immunohistochemical expression of IGF1 receptor (IGFR-I), IGF1 and IGF2 in 94 samples of GISTs. IGF1 and IGF2 expression was scored in three classes: negative (N), moderate (M) and strong (S), according to staining inte...