The composition of isolated natural organic matter (NOM) fractions was studied by means of high pressure size-exclusion chromatography (HP-SEC), specific ultraviolet absorbance (SUVA) at 254 nm, total luminescence spectroscopy (TLS) and synchronous scanning fluorescence measurements. The HPSEC, SUVA and TLS studies revealed that humic acids (HA) and hydrophobic acids (HOA) are composed of compl...

[2]Research Progress(10.5 points) In this project, we investigated the role of Nand C-terminal domain of human voltage-gated proton channel Hv1 in the gating mechanism using fluorescence technology. The plan in details is as follows: (1) To determine the interaction between Nand C-terminal domains, the N-terminal domain was cloned, expressed and purified. (2) The interaction between Nand C-term...

Interaction of flavokawain B (FB), a multitherapeutic flavonoid from Alpinia mutica with the major transport protein, human serum albumin (HSA), was investigated using different spectroscopic probes, i.e., intrinsic, synchronous, and three-dimensional (3-D) fluorescence, circular dichroism (CD), and molecular modeling studies. Values of binding parameters for FB-HSA interaction in terms of bind...

Cationic dye Acridine orange (AO) has wide applications especially in biologicalfields such as analysis of lysosomal and mitochondria content by flow cytometryand so on. In the current work, spectroscopy of acridine orange (AO) dye at bothlow concentrations (mdye/mwater=6.25×10-5, 3.12×10-5) and high concentrations(mdye/mwater=0.002, 0.001) was studied in confined water ...

Anthracyclines are considered to be some of the most effective anticancer drugs for cancer therapy. However, drug resistance and cardiotoxicity of anthracyclines limit their clinical application. An 3'-azido disaccharide analogue of daunorubicin, 7-[4-O-(2,6-dideoxy-3-O-methyl-alpha-l-arabino-hexopyranosyl)-3-azido-2,3,6-trideoxy-alpha-l-lyxo-hexopyranosyl]daunorubicinone (ADNR-3), was shown to...

In This research, gold nanoparticles were synthesized and functionalized by the antibody of aflatoxins. The quenching of the fluorescence of excitation emission matrices (EEM) of two type of aflatoxins (B1, G1), provoked by the gold nanoparticles, was studied by principal component analysis (PCA) and multivariate curve resolution with alternating least squares (MCR-ALS). These aflatoxins show q...

The binding of 1-chloro-3-(2-methyl-5-nitro-1H-imidazole-1-yl) propan-2-ol (Ornidazole) to human serum albumin (HSA) was studied by fluorescence and UV-visible spectroscopy. Interaction ornidazole (OR) with HSA identified Stern-Volmer Van’t Hoff equations. constant, Kb the thermodynamic parameters, ∆H, ∆S, ∆G at different temperatures were calculated several Data shows that quenching mechanism ...

In This research, gold nanoparticles were synthesized and functionalized by the antibody of aflatoxins. The quenching of the fluorescence of excitation emission matrices (EEM) of two type of aflatoxins (B1, G1), provoked by the gold nanoparticles, was studied by principal component analysis (PCA) and multivariate curve resolution with alternating least squares (MCR-ALS). These aflatoxins show q...