The histone-like protein HU is the most-abundant DNA-binding protein in bacteria. The HU protein non-specifically binds and bends DNA as a hetero- or homodimer, and can participate in DNA supercoiling and DNA condensation. It also takes part in DNA functions such as replication, recombination, and repair. HU does not recognize any specific sequences but shows a certain degree of specificity to ...

The molecular mechanism of the Diels–Alder reaction with participation cyclopentadiene and isopropyl 3-nitroprop-2-enate was examined based on wb97xd/6-311+G(d) (PCM) quantum chemical calculations. It found that type for conversion addends depends significantly conditions. In less-polar environments, a one-step polar is realised. more solvents, formation “extended”-type zwitterionic intermediat...

W e have previously shown that bestatin (an aminopeptidase inhibitor) permits the accumulation of diand tripeptide intermediates in the degradation of abnormal globin (Botbol, V., and Scornik, 0. A. (1979) Proc. Natl. Acad. Sei. U. S. A. 76, 710-713; Botbol, V., and Scornik, 0. A. (1979) J. Biol. Chem. 254, 11254-11257). We now report that this drug (1 mg, intravenously, 5-30 min) causes simila...

The generation and resolution of joint molecule recombination intermediates is required to ensure bipolar chromosome segregation during meiosis. During wild type meiosis in Caenorhabditis elegans, SPO-11-generated double stranded breaks are resolved to generate a single crossover per bivalent and the remaining recombination intermediates are resolved as noncrossovers. We discovered that early r...

P owerful tools for identifying reaction intermediates and byproducts of chemical reactions are crucial for the development of high-yield chemical syntheses, not only for testing theoretical mechanistic models and calculations, but also to obtain improved understanding of reaction mechanisms during trial experiments. This will enable more efficient steering toward optimum yields for a variety o...

Free radicals intermediates are formed on the surface of metal oxide semiconductors, namely Ti02 and Fe203, following the light-induced charge separation upon band-gap illu-mination. These radicals appear to adhere strongly to the ~.urfaces of the small colloidal particles (diameters between 5 and 20 nm) employed in this study. A second electron-transfer step yielding the observed end-products ...

It has long been suggested that existence of partially folded intermediates may be essential for proteins to fold in a biologically meaningful time scale. Although partially folded intermediates have been commonly observed in larger proteins, they are generally not detectable in the kinetic folding of smaller proteins (approximately 100 amino acids or less). Recent native-state hydrogen exchang...

Site-directed mutagenesis and Laue diffraction data to 2.5 A resolution were used to solve the structures of two sequential intermediates formed during the catalytic actions of isocitrate dehydrogenase. Both intermediates are distinct from the enzyme-substrate and enzyme-product complexes. Mutation of key catalytic residues changed the rate determining steps so that protein and substrate interm...

Native phytochrome (124kdalton) was isolated from etiolated seedlings o f Avena sativa L. cv. Pirol (Baywa, Munich). From low temperature spectra (0 °C to — 165°C ) o f phytochrom e samples irradiated either with red or far-red light, spectra o f intermediates were calculated. Both absorption bands o f the phytochrome chromophore were reported. On the pathway P r i V r , native phytochrome does...