Pfiesteria piscicida is a harmful bloom-forming alga that has received a great deal of attention due to its potential association with large fish kills and neurological problems in humans. Since the discovery of Pfiesteria, several other Pfiesteria-like dinoflagellates (PLDs) have also been identified. Genetic identification and phylogenetic relationships among the PLDs commonly utilize sequenc...

rRNA genes are attractive targets for developing PCR assays targeting human fungal pathogens. Most studies have focused on the 18S rRNA gene, internal transcribed spacers, and the 5' end of the 28S rRNA gene. An approximately 2,900-bp region of the 28S rRNA gene remains largely unexplored because sequences of many medically relevant fungi are either unavailable or undefined in genomic databases...

Panaeolus punjabensis M. Asif, Q. Firdous, A. Izhar, Niazi & Khalid sp. nov. was collected from three different localities (Bahawalnagar, Kasur, and Lahore) in Punjab, Pakistan. Morphological observations phylogenetic analyses based on nuclear encoded internal transcribed spacers (ITS1-5.8S-ITS2 = ITS) D1/D2 domain of large subunit (28S) rDNA confirmed the taxonomic distinctness this specie...

This study aimed to identify and characterize the coffee varieties cultivated in Nepal using molecular phylogenetic analysis. The identification genetic relationship of twenty five were collected from Coffee Development Center, Gulmi, Nepal. DNA was isolated leaf tissue, Internal Transcribed Spacers Region (ITS)-specific PCR performed, followed by sequencing tree construction. BLASTN performed ...

Immunoelectron microscopy and in situ hybridization have been used to investigate the precise location of transcribed and non-transcribed rDNA spacer sequences. Whereas a 5'-external transcribed spacer sequence is predominantly visualized in the fibrillar centers of nucleoli, a non-transcribed spacer sequence is preferentially detected in the interstices, in close contact with the fibrillar cen...

Trillions of microbes representing all kingdoms life are resident in, and on, humans holding essential roles for the host development physiology. The last decade over a dozen online tools servers, accessible via public domain, have been developed analysis bacterial sequences; however, fungi is still in its infancy. Here, we present web server dedicated to comprehensive human mycobiome (i) trans...